Stress, transport and metabolism of alpha-glycosides in Saccharomyces cerevisiae

• Grant number: 04/10067-6
• Support Opportunities: Research Projects - Thematic Grants
• Start date: January 01, 2005
• End date: August 31, 2009
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Pedro Soares de Araujo
• Grantee: Pedro Soares de Araujo
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The three research groups involved in this project have experience in collaborative work gained in a previous thematic project that lasted from 1996 to 2001. All the groups involved in this project were influenced by Prof. Anita Panek pioneer work that has studied trehalose effects on yeast metabolism since 1960. All participants have accumulated a great deal of expertise in this field in the last ten years. In this thematic project we intend to study cellular responses to stress conditions using the yeast Saccharomyces cerevisiae as a model organism for eukaryotic cells. We intend to explore the trehalose protective effects under stress conditions, focusing in oxidative stress. The tolerance acquisition mechanism under stress conditions that disrupt the cellular redox equilibrium will be our main interest in this research line. Since plasma membrane trehalose protection depends on the presence of the disaccharide on both leaflets of the membrane, the manipulation of the trehalose transporter transmembrane channel structure could lead us to dissociate the glucoside transport from the proton co-transport. Thus, the mutated gene could be expressed in other eukaryotic cell types, which would allow us to understand the conditions for dehydration resistance in those cell types. The study of the metabolic regulation for other alpha-glucosides besides trehalose could provide interesting results about the mechanism of his regulation, and eventually, led us to obtain strains that utilize these carbon sources more effectively. This could have some biotechnological interest. The mechanism of action of the purified and reconstituted into liposome's trehalose transporter will be studied both with the native as well as with the mutant proteins. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUVAL, E. H.; ALVES, JR., S. L.; DUNN, B.; SHERLOCK, G.; STAMBUK, B. U.. Microarray karyotyping of maltose-fermenting Saccharomyces yeasts with differing maltotriose utilization profiles reveals copy number variation in genes involved in maltose and maltotriose utilization. Journal of Applied Microbiology, v. 109, n. 1, p. 248-259, JUL 2010. (04/10067-6)

ALVES JÚNIOR, SERGIO L.; HERBERTS, RICARDO A.; HOLLATZ, CLAUDIA; TRICHEZ, DEBORA; MILETTI, LUIZ C.; ARAUJO, PEDRO S. DE; STAMBUK, BORIS U.. Molecular analysis of maltotriose active transport and fermentation by Saccharomyces cerevisiae reveals a determinant role for the AGT1 permease. Applied and Environmental Microbiology, v. 74, n. 5, p. 1494-1501, Mar. 2008. (04/10067-6)

ALBUQUERQUE, PATRÍCIA MELCHIONNA; WITT, MARIA ALICE; STAMBUK, BORIS UGARTE; NASCIMENTO, MARIA DA GRAÇA. Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent. Process Biochemistry, v. 42, n. 2, p. 141-147, Feb. 2007. (04/10067-6)

FERNANDES, PATRICIA N.; MANNARINO, SERGIO C.; SILVA, CARMELITA G.; PEREIRA, MARCOS D.; PANEK, ANITA D.; ELEUTHERIO, ELIS C. A.. Oxidative stress response in eukaryotes: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae. Redox Report, v. 12, n. 5, p. 236-244, Oct. 2007. (04/10067-6)

FRANÇA, M. B.; PANEK, A. D.; ELEUTHERIO, E. C. A.. Oxidative stress and its effects during dehydration. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 146, n. 4, p. 621-631, Apr. 2007. (04/10067-6)

SILVA, C. G.; RAULINO, R. J.; CERQUEIRA, D. M.; MANNARINO, S. C.; PEREIRA, M. D.; PANEK, A. D.; SILVA, J. F. M.; MENEZES, F. S.; ELEUTHERIO, E. C. A.. In vitro and in vivo determination of antioxidant activity and mode of action of isoquercitrin and Hyptis fasciculata. Phytomedicine, v. 16, n. 8, p. 761-767, AUG 2009. (04/10067-6)

ADAMIS, PAULA D. B.; PANEK, ANITA D.; ELEUTHERIO, ELIS C. A.. Vacuolar compartmentation of the cadmium-glutathione complex protects Saccharomyces cerevisiae from mutagenesis. Toxicology Letters, v. 173, n. 1, p. 1-7, Aug. 2007. (04/10067-6)

HERDEIRO R. S.; PEREIRA M. D.; PANEK A. D.; ELEUTHERIO E. C. A.. Trehalose protects Saccharomyces cerevisiae from lipid peroxidation during oxidative stress. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1760, n. 3, p. 340-346, Mar. 2006. (04/10067-6)

STAMBUK, BORIS U.; DUNN, BARBARA; ALVES, JR., SERGIO L.; DUVAL, EDUARDA H.; SHERLOCK, GAVIN. Industrial fuel ethanol yeasts contain adaptive copy number changes in genes involved in vitamin B1 and B6 biosynthesis. Genome Research, v. 19, n. 12, p. 2271-2278, DEC 2009. (04/10067-6)

SILVA, VANESSA DUTRA; STAMBUK, BORIS UGARTE; NASCIMENTO, MARIA DA GRACA. Efficient chemoselective biohydrogenation of 1,3-diary1-2-propen-1-ones catalyzed by Saccharomyces cerevisiae yeasts in biphasic system. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v. 63, n. 3-4, p. 157-163, MAY 2010. (04/10067-6)

CADETE, RAQUEL M.; SANTOS, RENATA O.; MELO, MONALIZA A.; MOURO, ADRIANE; GONCALVES, DAVI L.; STAMBUK, BORIS U.; GOMES, FATIMA C. O.; LACHANCE, MARC-ANDRE; ROSA, CARLOS A.. Spathaspora arborariae sp nov., a D-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Research, v. 9, n. 8, p. 1338-1342, Dec. 2009. (04/10067-6)

MANNARINO, SERGIO C.; VILELA, LEONARDO F.; BRASIL, ALINE A.; ARANHA, JULIANA N.; MORADAS-FERREIRA, PEDRO; PEREIRA, MARCOS D.; COSTA, VITOR; ELEUTHERIO, ELIS C. A.. Requirement of glutathione for Sod1 activation during lifespan extension. YEAST, v. 28, n. 1, p. 19-25, JAN 2011. (04/10067-6)

ADAMIS, PAULA D. B.; MANNARINO, SERGIO C.; RIGER, CRISTIANO J.; DUARTE, GISELLE; CRUZ, ANA; PEREIRA, MARCOS D.; ELEUTHERIO, ELIS C. A.. Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism. BIOMETALS, v. 22, n. 2, p. 243-249, APR 2009. (04/10067-6)

VIEIRA CASTRO, FREDERICO AUGUSTO; MARIANI, DIANA; PANEK, ANITA DOLLY; ARAUJO ELEUTHERIO, ELIS CRISTINA; PEREIRA, MARCOS DIAS. Cytotoxicity Mechanism of Two Naphthoquinones (Menadione and Plumbagin) in Saccharomyces cerevisiae. PLoS One, v. 3, n. 12, p. e3999, 2008. (04/10067-6)

BADOTTI‚ F.; DÁRIO‚ M.G.; ALVES‚ S.L.; CORDIOLI‚ M.L.A.; MILETTI‚ L.C.; DE ARAUJO‚ P.S.; STAMBUK‚ B.U.. Switching the mode of sucrose utilization by Saccharomyces cerevisiae. Microbial Cell Factories, v. 7, n. 1, p. 4, 2008. (04/10067-6)