The use of direct interphase fluorescence in situ hybridization (fish) on spontaneous abortion/stillborn tissue compared to standard cytogenetics.

• Grant number: 97/13368-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 1998
• End date: August 31, 1999
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Jose Fernando Pereira Arena
• Grantee: Jose Fernando Pereira Arena
• Host Institution: Faculdade de Medicina de Marília (FAMEMA). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). Marília , SP, Brazil

Abstract

This proposal initiates a genetic study of spontaneous abortions/ stillborns using standard cytogenetics and fluorescence in situ hybridization (fish) at the new Marilia genetics laboratory. Goals are to: 1) compare findings with previous studies by the principal investigator and others; 2) establish culture success rates in Marilia laboratory; 3) establish chromosome abnormality rates in the region; 4) evaluate if direct interphase fish is useful for screening trisomies and triploids in abortuses/ stillborns; 5) evaluate if direct interphase fish detects confined placental mosaicism; 6) develop computer-assisted karyotyping and color imaging in the Marilia laboratory as an aid to teaching, research and clinical service. (AU)