Genetic Polymorphism of the immune response in humans: studies by genotyping and gene expression analysis

• Grant number: 01/09850-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: June 01, 2002
• End date: July 31, 2006
• Field of knowledge: Biological Sciences - Immunology - Immunogenetics
• Principal Investigator: Anna Carla Renata Krepel Goldberg
• Grantee: Anna Carla Renata Krepel Goldberg
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Pesquisadores principais: Edecio Cunha Neto

Abstract

This study aims to analyse human genomic variability in a systematic way in order to identify genetic risk for multifactorial diseases and also evaluate its functional impact in a more individualized form. 1) a new family of genes called MIC was recently identified (MHC Class I Chain-related). MICA and MICB located next to the HLA-B locus, are functional and exhibit special biological characteristics. To this date 49 alleles for MIC-A and 11 MIC-B alleles have been described. Expression of these molecules is induced by cellular stress, and has been shown in normal and tumoral epithelial cells, in endothelium and in many others. To evaluate the importance of MIC in immune response we will compare allele distribution in a sample of the normal São Paulo population with patients with Behçet disease, besides measuring MIC-A gene expression in epithelial cell lines from different tumor stages; 2) in a second project we will analise the role of genetic polymorphism of cytokines involved in immune response to disease comparing functional and clinical data with SNP genotyping and gene expression of candidate genes by RT -PCR and microarray analysis in Chagas' disease cardiomyopathy, Rheumatic Heart Disease and chronic rejection in kidney and heart transplant patients, ali are major research projects in our laboratory. Due to the nature of these studies and the quantity of information already at hand, it will be necessary to obtain and classify genetic data referring to normal individuals, in order to integrate information into the DNA bank of the laboratory. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BITTENCOURT, PAULO LISBOA; CARNEVALE MARIN, MARIA LUCIA; COUTO, CLAUDIA ALVES; RACHID CANCADO, EDUARDO LUIZ; CARRILHO, FLAIR JOSE; GOLDBERG, ANNA CARLA. Analysis of HFE and non-HFE gene mutations in Brazilian patients with hemochromatosis. Clinics, v. 64, n. 9, p. 837-841, 2009. (01/09850-0)

LÉA CAMPOS DE OLIVEIRA; RAJENDRANATH RAMASAWMY; JAILA DIAS BORGES; MARIA LUCIA CARNEVALE MARIN; NATALIE GUIDA MULLER; JORGE KALIL; ANNA CARLA GOLDBERG. Frequência de polimorfismo de nucleotídeo único de alguns genes da resposta imune em amostra populacional da cidade de São Paulo, Brasil. Einstein (São Paulo), v. 9, n. 3, p. 359-366, 2011-09-00. (01/09850-0, 05/00553-3)

VERÔNICA PORTO CARREIRO DE VASCONCELLOS COELHO; RAFAEL IOSCHPE; CRISTINA CALDAS; MONICA SPADAFORA-FERREIRA; JOÃO AMERICO FONSECA; MARIA REGINA ALVES CARDOSO; SELMA ALIOTTI PALACIOS; JORGE KALIL; ANNA CARLA GOLDBERG. Papéis contrastantes das variantes polimórficas dos genes TGFB1 e IFNG do doador e do receptor na rejeição crônica de transplantados renais. Einstein (São Paulo), v. 9, n. 1, p. 46-51, 2011-03-00. (01/09850-0)