Periodontal disease associated with Down syndrome: expression analysis of immune response genes

• Grant number: 05/00588-1
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2005
• End date: June 30, 2009
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Raquel Mantuaneli Scarel Caminaga
• Grantee: Raquel Mantuaneli Scarel Caminaga
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 05/03175-0 - Periodontal disease associated with Down syndrome: expression analysis of immune response genes, BP.TT

Abstract

Many periodontopathic bacteria may early colonize the oral cavity of Down syndrome (DS) individuals. It is demonstrated in the high periodontal disease (PD) prevalence (40%) in DS adolescents. This number increases up to 100% in nearly 30 years old individual. The poor oral hygiene of the DS cannot explain by itself severe periodontal destruction in those persons. It also is observed low chemotaxis and phagocytosis function, in despite of the normal number of neutrophil and monocytes cells. DS individuals present superoxide dismutase enzyme superexpression that diminishes the capacity of the polymorphonuclear cells against microorganisms. There is a lot to investigate about the immune system influence at greater susceptibility to PD in DS individuals, due to the short of studies about cytokine expression and its receptors. Important cytokines, like Interferon-g (IFN-g) which represents the Th1 response have not been very studied in DS individuals with PD. Likewise Interleukin 10 (IL10), which represents the Th2, seems that have never been studied in those individuals. Both IL10 and IFN-g have two receptors each. The IL10RB and IFGR2 are localized on the chromosome 21 (21q), a highly conserved region between humans and mice. The IFN-g bond to the receptors activates the STAT1 (Signal Transduction and Activator of Transcription). It controls the synthesis of microbicidal substances like iNOS (inducible Nitric Oxide Synthase) by the catalysis of the nitric oxide gas. The superoxide dismutase (SOD) is another important enzyme whose gene is located in the chromosome 21. DS individuals show intracellular high concentration of peroxide hydrogen due to the SOD1 superexpression, which converts superoxide into peroxide hydrogen. The aim of this study is to investigate the IL10, IL10RA, IL10RB, IFN-g, IFNGR1, IFNGR2, STAT1, iNOS e SOD1 gene expression in DS individuals with and without PD. Besides, the NO production will be investigated by the concentration of nitrate and nitrite measurement. These data will be compared with the others obtained from non-syndromic individuals (controls) with and without PD. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TANAKA, MARCIA H.; GIRO, ELISA M. A.; CAVALCANTE, LICIA B.; PIRES, JULIANA R.; APPONI, LUCIANO H.; VALENTINI, SANDRO R.; SPOLIDORIO, DENISE M. P.; CAPELA, MARISA V.; ROSSA, JR., CARLOS; SCAREL-CAMINAGA, RAQUEL M.. Expression of interferon-gamma, interferon-alpha and related genes in individuals with Down syndrome and periodontitis. CYTOKINE, v. 60, n. 3, p. 875-881, DEC 2012. (05/00588-1, 05/03175-0)

CAVALCANTE, LICIA BEZERRA; TANAKA, MARCIA HIROMI; PIRES, JULIANA RICO; APPONI, LUCIANO HENRIQUE; APARECIDA GIRO, ELISA MARIA; VALENTINI, SANDRO ROBERTO; PALOMARI SPOLIDORIO, DENISE M.; CAPELA, MARISA VEIGA; ROSSA, JR., CARLOS; SCAREL-CAMINAGA, RAQUEL M.. Expression of the Interleukin-10 Signaling Pathway Genes in Individuals With Down Syndrome and Periodontitis. Journal of Periodontology, v. 83, n. 7, p. 926-935, JUL 2012. (05/00588-1, 05/03175-0)