Study of proteins from hemolymph of Lonomia obliqua with potential antiviral action

• Grant number: 06/02108-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2006
• End date: July 31, 2009
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ronaldo Zucatelli Mendonca
• Grantee: Ronaldo Zucatelli Mendonca
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The control of viroses human, in special the induced for the Influenza virus, is of high interest in the human and animal health. However, few agents are available for the control of these diseases. For this reason, identification of agents with antiviral action is of higgest importance. Recently, we have demonstrated(Petricevich VL and Mendonça RZ) the presence of proteins with potential antiviral effect against the measles virus in the poison of Crotalus durissus terrificus. Moreover, we have also observed (FAPESP 01/09040-8/) the probable existence of a protein with action antiviral in the Lonomia obliqua hemolimph.Some studies he has been carried through demonstrating the presence of biological activy proteins in the hemolimph showing enzymatic effect (Shiotsuki et al 2000;Yamamoto et al 1999; Guerrero et al, 1999; Jiang et al, 1999; Rosenfeld and Vanderberg, 1998; Hamdaoui et al 1998), hormonais (Huberman et al 2000; Lin et al 1998; Kurata, et al, 1994; Jones, et al, 1993), with immune action, antibactericidal and antifungica (Zhu et al 2000; Lowenberger et al 1999; Lambert et al 1999; Gross et al 1998; Johns et al 1998; Lans-Mendoza, et al, 1996) and antifreezing (Peters, et al, 1993).The spite of this, few works has been carried through demonstrating the effect of hemolimph in cell culture.The first works demonstrating effect in the cellular growth and time of survival cellular had been carried through in the decada 50 and 60 (Wyatt, 1956; Grace, 1958; 1962 and 1967). However, after these pioneering works very little were carried through in this direction; By the other hand, the use of hemolimph in insect cell culture has was substituted by bovine fetal serum. Very recently some works have appeared demonstrating the positive effect of hemolimph in the promotion of viral growth (Rhee and Park, 2000; Rhee et al 1999, Kanaya and Kobayashi 2000, Maranga et al, 2003) and recombinant protein production (Woo et al 1997, Mendonça 2006). Kanaya and Kobayashi (2000), had isolated and characterized one protein in the silkworm hemolimph which increase the viral heading in 10.000. However, few studies he has been carried through searching to isolate and to characterize the involved factors in these effect (Nussbaumer, et al 2000; Shishikura, et al, 1997 and 1996; Moon et al 1995; Ochanda, 1992).The spite of this variety of activities observed in hemolimph, few works has been carried through searching to identify proteins with antiviral action in the insects hemolymph (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRECO, KATIA N.; MENDONCA, RITA MARIA Z.; MORAES, ROBERTO H. P.; MANCINI, DALVA. A. P.; MENDONCA, RONALDO Z.. Antiviral activity of the hemolymph of Lonomia obliqua (Lepidoptera: Saturniidae). Antiviral Research, v. 84, n. 1, p. 84-90, OCT 2009. (06/02108-0)