New biophysical model for simulation of rat cardiac muscle fiber strength considering the excitation-contraction-relaxation coupling (E-C-R)

• Grant number: 06/02830-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2007
• End date: March 31, 2009
• Field of knowledge: Biological Sciences - Biophysics - Biophysics of Processes and Systems
• Principal Investigator: Daniel Gustavo Goroso
• Grantee: Daniel Gustavo Goroso
• Host Institution: Pró-Reitoria de Pesquisa, Pós-Graduação e Extensão e Assuntos Comunitários. Universidade de Mogi das Cruzes (UMC). Mogi das Cruzes , SP, Brazil

Abstract

In this project, the cardiac muscle fiber strength will be modeled using a set of differential equations. The modeling will consider some theories and experimental data described in previous studies, in order to obtain stable simulations of cardiac cell E-C-R dynamics. It is proposed the simulation of models previously described in the literature in order to get insights for the development and tests of a new model in which it will be considered the ECR coupling effect and the viscous-elastic effect, being the latter an important characteristic of the new model. The coupled equation system that describes the model will be implemented in Visual C++ and represents the following biophysical dynamics: 1) L-type Ca2+ current (ICaL); 2) Calcium and other ions movements through the sarcoplasm (Fast inward Na+ current: INa; Slow Na+ current: IL-Na. Delayed rectifier K+ current fast an slow: IKs e IKf ); 3) Calcium efflux across the sarcolemma by Na+-Ca2+ exchange (INa-Ca); 4) Calcium uptake by SR Ca2+-ATPase (Iserca); 5) Calcium buffers dynamics (I[CaBu]); 6) Ca2+ movement mechanisms (Ca2+ influx across the sarcolemma and Ca2+ movement through SRRC and 7) Viscous-elastic mechanism for strength generation. The model will be validated by a series of experiments described in the literature (Bassani e Bers, 1995; Bassani et al., 1995; Bers et al., 1990; Cheng et al., 1996; Fabiato, 1985; Gilchrist et al., 1992; Ginsburg et al., 1998; Shannon e Bers, 1997; Wier et al., 1994). It is expected that the new model will be a useful tool for the investigation of strength on isolated atrium and isolated heart in isometric condition. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, ROBSON R.; GOROSO, DANIEL G.; BISSACO, MARCIA A. S.. MioLab: Simulator for cardiac myocyte contractile force of rat based on the dynamics of calcium. MEDICAL ENGINEERING & PHYSICS, v. 35, n. 3, p. 338-349, MAR 2013. (06/02830-7)

DA SILVA, ROBSON RODRIGUES; SILVA BISSACO, MARCIA APARECIDA; GOROSO, DANIEL GUSTAVO. MioLab, a rat cardiac contractile force simulator: Applications to teaching cardiac cell physiology and biophysics. COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, v. 122, n. 3, p. 480-490, DEC 2015. (13/20220-5, 06/02830-7)