The Sleep is an important factor for the maintenance of health, acting in processes of restoration and conservation of energy, memory consolidation, protection of organic and physiological functioning of the immune system, and cardiovascular health. However, animal models suggest that alterations caused by sleep restriction/ deprivation are associated with significant losses in specific hormonal axis, like as a increase in corticosterone, sympathetic activity, thyroid hormones and decreases of testosterone, GH, IGF-1. This set of changes essentially establishes a catabolic environment that can cause a series of physiological damage that can be perceived both in skeletal muscle tissue and in cardiac tissue. In muscle tissue, this catabolic environment may promote the process of muscle atrophy. Already in the cardiac tissue, some of these changes may be related to changes in the route of an important intracellular messenger, Ca2 +, thereby interfering with activities such as contractility, metabolism, transport, transcription and secretion. Among the strategies used to minimize this condition, the practice of physical exercise, has been establish. Particularly resistance exercise, has gained attention because of the important physiological adaptations resulting from this activity, highlighting the metabolic and hormonal changes of intracellular events beyond themselves, which alone or integrated may account for increases in strength, the cross-sectional area and increasing or maintaining muscle protein synthesis and physiological cardiac hypertrophy (concentric). Considering the potential effect of resistive exercise on skeletal muscle and heart, the main of this study is to evaluate the effects of resistance training on skeletal and cardiac muscle of rats submitted to paradoxical sleep deprivation for 96 hours (PSP96). Thus, will be used male Wistar rats, which will be submitted to a protocol of high intensity resistance training for 8 weeks, followed by a protocol of paradoxical sleep deprivation for 96 hours by the method of modified multiple platforms. After these procedures the animals will be euthanized, blood, gastrocnemius muscle and cardiac muscle are removed for histological and biochemical analyzes. For evaluation of anabolic and catabolic hormone responses will be measured levels of testosterone, IGF-1, Corticosterone and thyroid hormones. Histological analysis of skeletal muscle and cardiac muscle will be done with hematoxylin and eosin (HE) and ATPase (skeletal muscle) to identify the cross-sectional area of the fiber and the diameter of cardiac myocytes and identification of skeletal muscle fiber type. Biochemical analysis will be performed by Western Blotting of proteins keys of synthesis pathways and protein degradation, like as AKT, mTOR, p70s6K1, FOXO, MuRF1 and myostatin and proteins involved in the calcium pathway, PLB and SERCA2 and their active forms. The basal metabolism and cardiac functional assessment will be conducted for the purpose of completion of the study. The study has the approval of the Ethics Committee of the Universidade Federal de São Paulo / Hospital São Paulo, No. 0764/10. (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
MOREIRA ANTUNES, HANNA KAREN;
LEE, KIL SUN;
PHILLIPS, STUART M.;
DE CAMPOS GIAMPA, SARA QUAGLIA;
SOUZA, HELTON DE SA;
DE MORAES, WILSON MAX;
DE MELLO, MARCO TULIO.
Resistance training minimizes catabolic effects induced by sleep deprivation in rats.
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM,
Web of Science Citations: 11.