Effects of sleep deprivation on basal metabolic rate of Wistar rats

• Grant number: 12/07033-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2012
• Effective date (End): December 31, 2012
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Hanna Karen Moreira Antunes
• Grantee: Pedro Henrique dos Santos Prado
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Sleep is a very important process to sustain health, many physiological functions and cognitive condition. However, animal models suggest that sleep deprivation changes, as well as sleep restriction, can generate some significant deficits over specifics hormonal axes, such a very large increase on corticosterone secretion and the reduction of testosterone, growth hormone (GH) and insulin-like growth factor (IGF-1), establishing an essentially catabolic environment which allows loss of muscle mass and total body weight that can cause changes on basal metabolic rate. Therefore, the main objective of this study is evaluate the effects of paradoxical sleep deprivation (PSD) over metabolism of rats submitted to 96 hours sleep deprivation protocol (PSP96h), observing the hormonal catabolic and anabolic responses and the possible changes over the histomorphometry of skeletal muscle. For this purpose, we will use male rats Wistar, categorized in three groups: control (CTRL), sham (SHAM) and sleep-deprived (PSP96h). The sleep-deprived group will be measured for basal metabolic rate following a protocol of paradoxical sleep deprivation for 96 hours by the modified multiple platform method with subsequent re-analysis of metabolism. After these procedures the animals will be euthanized, blood and tibialis anterior muscle are removed for histological and blood analysis. For the evaluation of hormonal responses, we will measure the blood levels of testosterone, corticosterone, IGF-1, GH and thyroid hormones. The histological analysis of tibialis anterior muscle will be done with hematoxylin and eosin (HE) for histomorphometry. (AU)