Anesthesia can alter the levels of corticosterone and the phosphorylation of signaling molecules

Author(s)

Pereira, Gabriela Cruz ^[1] ; Neto, Marcos Monico ^[2] ; Moreira Antunes, Hanna Karen ^[2] ; Lee, Kil Sun ^[1] ; Mello da Luz, Marcio Henrique ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biochem, Rua Pedro de Toledo 669, 8 Andar, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BMC RESEARCH NOTES; v. 14, n. 1 SEP 19 2021.
• Web of Science Citations: 0

Abstract

Objective Neuroscience research using laboratory animals has increased over the years for a number of reasons. Some of these studies require the use of anesthetics for surgical procedures. However, the use of anesthetics promotes several physiological changes that may interfere with experimental results. Although the anesthetics and methods of delivery used to vary, one of the most common is ketamine associated with another compound such as xylazine. We aimed to evaluate the effect of ketamine and xylazine (KX) on corticosterone levels and on the degree of phosphorylation of p44/42 (ERK1/2), Src kinases and calcium/calmodulin-dependent kinase II (CAMKII). We also compared the effects of KX on sleep deprivation, which is known to affect the hormonal profile including corticosterone. Results We found that the use of KX can increase corticosterone levels and alter the degree of phosphorylation of signaling proteins. (AU)

• FAPESP's process: 17/10404-2 - Evaluation of sleep deprivation effects on function and metabolism of amyloid-beta peptides
• Grantee: Márcio Henrique Mello da Luz
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/00152-5 - Effect of resistance exercise in cardiac and skeletal muscle of Wistar rats submitted to paradoxical sleep deprivation
• Grantee: Hanna Karen Moreira Antunes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15962-7 - Effects of resistance exercise on trophic and atrophic patways in skeletal muscle of rats with paradoxal sleep deprivation
• Grantee: Marcos Mônico Neto
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/04297-6 - Impacts of nutrition and sleep on expression, solubility and functions of neural proteins.
• Grantee: Kil Sun Lee
• Support Opportunities: Regular Research Grants