Fluoride uptake by dental biofilm and biofilm fluid after the use of conventional and low-fluoride dentifrices in communities with different levels of fluoride in the drinking wate

• Grant number: 06/07258-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2007
• End date: October 31, 2009
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Marília Afonso Rabelo Buzalaf
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of the present study is to evaluate fluoride (F) uptake by dental biofilm and biofilm fluid after the use of conventional and low-F dentifrices, in communities with water [F]s of 0.04, 0.85 and 3.5 µg/mL. In the first phase, [F] and [Ca] present in saliva and dental biofilm will be measured 7 days after the use of dentifrices containing 0, 550 and 1,100 µgF/g (NaF). Children (n = 20 in each community) will receive a dental prophylaxis and scaling before the beginning of each experimental period and will brush their teeth twice daily, with the dentifrice provided, in a double blind, crossover design. Saliva and biofilm samples will be collected on the 7th day, 1 and 12 after the last use of the dentifrices. The children will receive a tube of a dentifrice not used in the previous week and the protocol will be repeated for 2 additional weeks. The second and third phases will be conducted only in the 0.85 µg/mL F community. The second phase will be conducted simultaneously to the first phase, and the samples collected will be analyzed for [F] and [Ca] in biofilm fluid also, in addition to whole biofilm and saliva, as described previously. The third phase will evaluate fluoride uptake in serial sections of the biofilm formed in situ. Leeds in situ devices for biofilm accumulation will be fixed on the buccal surfaces of the upper first molars and will be removed 7 days after the use of the dentifrices, 1 and 12 h after the last brushing. [F] will be determined after HMDS-facilitated diffusion for saliva samples; after buffering the sample with TISAB III and with the inverted electrode device for biofilm fluid samples; and after extraction with HClO4 and buffering with TISAB I for whole biofilm and serial sections of biofilm. [Ca] will be determined by AAS, for whole biofilm and serial sections of biofilm and using a micro-electrode, for biofilm fluid samples. Data will be analyzed by ANOVA and Tukey’s test and Linear Regression Analysis (p<0,05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PESSAN, J. P.; ALVES, K. M. R. P.; RAMIRES, I.; TAGA, M. F. L.; SAMPAIO, F. C.; WHITFORD, G. M.; BUZALAF, M. A. R.. Effects of Regular and Low-fluoride Dentifrices on Plaque Fluoride. JOURNAL OF DENTAL RESEARCH, v. 89, n. 10, p. 1106-1110, OCT 2010. (06/07258-0)

PESSAN, JULIANO PELIM; MANARELLI, MICHELE MAURICIO; KONDO, KARINA YURI; ITALIANI, FLAVIA DE MORAES; NAKAI KOBAYASHI, CLAUDIA AYUMI; WHITFORD, GARY MILTON; BOTAZZO DELBEM, ALBERTO CARLOS; RABELO BUZALAF, MARILIA AFONSO. Plaque fluoride concentrations associated to the use of conventional and low-fluoride dentifrices. AMERICAN JOURNAL OF DENTISTRY, v. 26, n. 6, p. 347-350, DEC 2013. (06/07258-0)