Production of enzymes and chelants by basidiomycetes and the relationship with the degradation of recalcitrant compounds

• Grant number: 07/00993-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2007
• End date: August 31, 2009
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Adriane Maria Ferreira Milagres
• Grantee: Adriane Maria Ferreira Milagres
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

The study of the oxidative mechanisms displayed by white-rot fungi for lignin biodegradation is important to assess the complete potential application of their lignin-degrading enzymes (LiP, MnP and laccase) as the biodegradation of several pollutants by these fungi has been directly correlated to their ability to degrade lignin. In this context, aiming to obtain better knowledge of their oxidative system in the absence of lignin-peroxidase, we will study the synergistic action between Fe3+-reducing activity and the lignin-degrading enzymes MnP and Lac for degradation of the dye azure B, which is not directly oxidized by MnP or Lac. Degradation of the dye will be studied using low molecular weight fractions (< 5 kDa) with Fe3+-reduction activity and high molecular weight fractions (>5 kDa) with manganese-peroxidase and laccase activities isolated from white-rot fungi cultures. In addition, the synergistic action between Fe3+-reducing activity-Fe3+/H2O2 and the lignin-degrading enzymes MnP and laccase, and also the relevance of this synergism as an oxidative pathway for lignin biodegradation by white-rot fungi will be discussed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUSSATTO, SOLANGE I.; DRAGONE, GIULIANO; FERNANDES, MARCELA; MILAGRES, ADRIANE M. F.; ROBERTO, INES C.. The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer's spent grain. Cellulose, v. 15, n. 5, p. 711-721, 2008. (07/00993-9)

ARANTES, VALDEIR; MILAGRES, ADRIANE M. F.; FILLEY, TIMOTHY R.; GOODELL, BARRY. Lignocellulosic polysaccharides and lignin degradation by wood decay fungi: the relevance of nonenzymatic Fenton-based reactions. Journal of Industrial Microbiology & Biotechnology, v. 38, n. 4, p. 541-555, APR 2011. (07/00993-9)