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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer's spent grain

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Author(s):
Mussatto, Solange I. [1, 2] ; Dragone, Giuliano [2] ; Fernandes, Marcela [2] ; Milagres, Adriane M. F. [2] ; Roberto, Ines C. [2]
Total Authors: 5
Affiliation:
[1] Univ Minho, Dept Engn Biol, P-4710057 Braga - Portugal
[2] Univ Sao Paulo, Dept Biotecnol, Escola Engn Lorena, Lorena, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Cellulose; v. 15, n. 5, p. 711-721, 2008.
Field of knowledge: Biological Sciences - Microbiology
Web of Science Citations: 41
Abstract

Brewer’s spent grain components (cellulose, hemicellulose and lignin) were fractionated in a two-step chemical pretreatment process using dilute sulfuric acid and sodium hydroxide solutions. The cellulose pulp produced was hydrolyzed with a cellulolytic complex, Celluclast 1.5 L, at 45 °C to convert the cellulose into glucose. Several conditions were examined: agitation speed (100, 150 and 200 rpm), enzyme loading (5, 25 and 45 FPU/g substrate), and substrate concentration (2, 5 and 8% w/v), according to a 23 full factorial design aiming to maximize the glucose yield. The obtained results were interpreted by analysis of variance and response surface methodology. The optimal conditions for enzymatic hydrolysis of brewer’s spent grain were identified as 100 rpm, 45 FPU/g and 2% w/v substrate. Under these conditions, a glucose yield of 93.1% and a cellulose conversion (into glucose and cellobiose) of 99.4% was achieved. The easiness of glucose release from BSG makes this substrate a raw material with great potential to be used in bioconversion processes. (AU)

FAPESP's process: 07/00993-9 - Production of enzymes and chelants by basidiomycetes and the relationship with the degradation of recalcitrant compounds
Grantee:Adriane Maria Ferreira Milagres
Support type: Regular Research Grants