Utilization of DNA-Stable Isotope Probing (DNA-SIP) technique for identification of autotrophic denitrifying microorganisms in sewage treatment systems using sulfide as electron donor

• Grant number: 10/03286-4
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2010
• End date: December 31, 2012
• Field of knowledge: Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
• Principal Investigator: Eugenio Foresti
• Grantee: Eugenio Foresti
• Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The aim of this research is to get insight into microorganisms involved in autotrophic denitrification using sulfide as electron donor. For this, the stable-isotope probing technique (DNA-SIP) will be applied. Firstly, autotrophic denitrifier enrichment culture feed with bicarbonate, sulphide and nitrate will be obtained from a wastewater system operated in pilot scale. Denitrification will be followed in time by GC and HPLC methods. To identify the metabolically active microorganisms involved in this metabolism, actively growing autotrophic denitrifiers culture will be cultivated in medium with 13C-labelled bicarbonate, sodium sulfide and sodium nitrate. CsTFA separation of 13C-labelled DNA will be followed by PCR amplification DGGE analysis and sequencing of 16S DNA genes from microbial populations involved with 13C-bicarbonate metabolism. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAIA, FLAVIA TALARICO; SOUZA, THEO S. O.; DELGADO DUARTE, RUBENS TADEU; POZZI, ELOISA; FONSECA, DEBORA; FORESTI, EUGENIO. Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment. Bioprocess and Biosystems Engineering, v. 39, n. 2, p. 341-352, FEB 2016. (10/01735-6, 10/03286-4)