Bixin-induced hepatoma cell lines as in vitro model for drug development

• Grant number: 09/54375-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2010
• End date: January 31, 2012
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Terezinha de Jesus Andreoli Pinto
• Grantee: Terezinha de Jesus Andreoli Pinto
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the preclinical stage of drug development, several in vitro models have been used as an alternative to the use of laboratory animals. The primary hepatocytes culture is one of the widely employed in vitro models for citotoxicity and drug metabolism studies, however the hepatoma cell lines can also be considered a good alternative if used in enzyme-induced state. The carotenoid bixin, extracted from urucum showed in vivo studies the capacity to induce some isoforms of the cytochrome P450 system, the main enzyme system involved in drug metabolism. In this work, bixin will be employed as inducer of cytochrome P450 system in HepG2 and Huh7 hepatoma cell lines. The bixin induced cell lines will be assessment as in vitro model in citotoxicity and drug metabolism studies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAKURAGUI MATUO, MIRIAM CRISTINA; DE OLIVEIRA TAKAMOTO, RAFAEL TERUITI; KIKUCHI, IRENE SATIKO; ANDREOLI PINTO, TEREZINHA DE JESUS. Effect of bixin and norbixin on the expression of cytochrome P450 in HepG2 cell line. Cell Biology International, v. 37, n. 8, p. 843-848, AUG 2013. (09/54375-0)