| Grant number: | 07/04148-1 |
| Support Opportunities: | Regular Research Grants |
| Start date: | December 01, 2007 |
| End date: | May 31, 2010 |
| Field of knowledge: | Health Sciences - Dentistry |
| Principal Investigator: | Fabio Daumas Nunes |
| Grantee: | Fabio Daumas Nunes |
| Host Institution: | Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
| Associated researchers: | José Mauro Granjeiro ; Katiúcia Batista da Silva Paiva ; Mari Cleide Sogayar |
| Associated scholarship(s): | 09/05293-0 - Temporal and spatial genes expression (Ras, Myc, Fos, Jun and Msx) and their transcription factors regulated by matrix metalloproteinases, their tissue inhibitors and RECK during mouse endochondral ossification, BP.TT |
Abstract
MMPs are zinc-dependent endopeptidases that, collectivelly, degrade all components of the ECM. They are able to remodelate the ECM during normal developmental processes such as embryogenesis and organogenesis, as well as in pathological processes such as tumoral invasion. The biological mineralization research looking for discovering the genes involved in the molecular mechanisms that control the endochondral ossification process. MMPs and their inhibitors (TIMPs and RECK) are responsable for bone matrix remodeling and, probably, determinate the level of its turnover. Msx2 is a homeobox-contains gene important for a limb development. Thus, our goal is evaluating the temporal-spatial genes expression (Ras, Myc, Jun, Fos and Msx2) and their transcription factors regulated by RECK, MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2, TIMP-3 and TIMP-4 in mice embryos and newborns during endochondral ossification by molecular biology (in situ hybridization and Real-Time PCR), immunoassay (immunohistochemistry and western blot), and biochemistry techniques (gelatin zymography). (AU)
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