Bovine sperm DNA: are there any changes at different ages?

• Grant number: 10/07599-7
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2010
• End date: June 30, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marion Burkhardt de Koivisto
• Grantee: Marion Burkhardt de Koivisto
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Janaina Torres Carreira ; Sílvia Helena Venturoli Perri ; Valéria Marçal Felix de Lima

Abstract

The integrity of the spermatic DNA is extremely important for effective production of healthy offspring. Several techniques provide access to the integrity of the chromatin, allowing the evaluation of possible risk of impaired fertility or problems in the embryonic and fetal development. The general objectives of this research project are to test the hypothesis that the capacity and quality of packing of chromatin, its integrity and the damage caused by free radicals are different in a group of young Nelore bulls, adult and senile. To get these answers 70 Nelore bulls will be assessed, with 18 to 156 months, divided into seven groups classified by age. The thawed semen will be evaluated on: the physical parameters (motility, vigor, morphology), acrossome and plasma membrane integrity; histones substitution by protamines measured with CMA3 stainning; SCSA® - Sperm Chromatin Structure Analyses and TUNEL method; nucleotide oxidization by the concentration of 8-oxoguanine. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KIPPER, B. H.; TREVIZAN, J. T.; CARREIRA, J. T.; CARVALHO, I. R.; MINGOTI, G. Z.; BELETTI, M. E.; PERRI, S. H. V.; FRANCISCATO, D. A.; PIERUCCI, J. C.; KOIVISTO, M. B.. Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF. Theriogenology, v. 87, p. 154-160, JAN 1 2017. (12/10471-8, 10/07599-7)

CARREIRA, J. T.; TREVIZAN, J. T.; CARVALHO, I. R.; KIPPER, B.; RODRIGUES, L. H.; SILVA, C.; PERRI, S. H. V.; DREVET, J. R.; KOIVISTO, M. B.. Does sperm quality and DNA integrity differ in cryopreserved semen samples from young, adult, and aged Nellore bulls?. BASIC AND CLINICAL ANDROLOGY, v. 27, 2017. (10/07599-7)