Transdentinal effects of different types of LED on odontoblast-like cells culture

• Grant number: 10/50798-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2010
• End date: June 30, 2012
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Carlos Alberto de Souza Costa
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The cellular biostimulation has been obtained by irradiating tissue with different types of light as LED. Recent research has shown that irradiation transdentinal pulp results in increased synthesis of dentin matrix and to a lesser intensity in the local inflammatory reaction. However, the mechanism of controlling this process remains unknown. The objective of this research paper will be to investigate the action of LED at three wavelengths on the metabolism of odontoblast cells cultured on 0.2 mm thick dentine discs. The odontoblast cell line MDPC-23 will be used and cultivated on the occlusal surface of dentin discs obtained from healthy human teeth. These discs will be mounted on an artificial pulp chamber developed for this study. LED will be put on discs to assess their possible transdentinal biostimulation. For evaluation of cellular metabolism, total protein and alkaline phosphatase activity of cells will be performed MTT assays, Read, Northcote and Alkaline Phosphatase Kit - Test for End Point, respectively. RT-PCR test will be performed to evaluate the expression of genes (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA TURRIONI, ANA PAULA; DE OLIVEIRA, CAMILA FAVERO; BASSO, FERNANDA GONCALVES; MORIYAMA, LILIAN TAN; KURACHI, CRISTINA; HEBLING, JOSIMERI; BAGNATO, VANDERLEI S.; DE SOUZA COSTA, CARLOS ALBERTO. Correlation between light transmission and permeability of human dentin. Lasers in Medical Science, v. 27, n. 1, p. 191-196, JAN 2012. (09/03615-0, 10/50798-0)