Set-up and standardization of human osteoblast and osteoclast cell cultures as tools for the study of the mechanisms involved in the alveolar bone loss secondary to periodontitis: the role of platelets

• Grant number: 09/15159-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2010
• End date: February 29, 2012
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Luis Carlos Spolidorio
• Grantee: Luis Carlos Spolidorio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The bone is a dynamic tissue under constant turn-over regulated by differentiation and activity of cells such as osteoblasts and osteoclasts, modulated by a wide variety of hormones, inflammatory mediators and growth factors. These mediators are found in periodontium (gingival and bone) secreted by a wide range of cells, such as monocytes, macrophages and platelets, being the last an important cell rich in variety of substances, whose analysis can be useful to correlate with the oral health status.In this way, the aim of this project is to investigate whether the plasm rich platelets may interfere with the mechanisms of bone resorption, either through a direct interaction with osteoclasts from healthy individuals or patients with periodontal disease or indirectly through osteoblasts, and if these events are related to the clinical status of patients with periodontal disease. In this study, we will analyze the effects of platelets rich plasm on the differentiation and activity (bone resorption) of of osteoclast precursors in vitro, using pure osteoclast cell cultures obtained from human peripheral blood monocytes, co-cultures with human osteoblasts and the intracellular mechanisms involved, through the analysis of protein and gene expression of some mediators (such as TNFa, interleukins, NFK², Akt, RANTES, MCP-1 and growth factors). (AU)