Molecular signatures of mesenchymal stem cell transcriptome from adipose tissue in differentiation mechanism: a special look for inflammation

• Grant number: 19/09943-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2019
• Effective date (End): November 04, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Willian Fernando Zambuzzi
• Grantee: Guilherme Gazolla Santana
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration, AP.JP

Abstract

The cell differentiation of bone cells from mesenchymal stem cells is still a mystery within area, especially for cells in compromising cells with adipose, muscular, cartilaginous and bone tissues. When do is the time to decide? First, the bone regeneration mechanism inflammation processes displays a major role and take importance up to first week of bone injury, presenting as an inflammatory tissue called a clot. The inflammatory response is dynamic and orchestrated by the release of active signaling molecules such as FGF-2, interleukin-1 (IL-1), IL6 and macrophage colony stimulating factor (MCSF), the latter molecule closely involved with biological processes of osteoclastogenesis. The inflammatory processes are governed by supramolecular structures called inflammossoma, structures capable of modulating the molecular processing of important interleukins. Previously unpublished data from our group, has been related a higher incidence of adipocytes instead osteoblast in ASC KO mice. Thus, we are looking for to understand better this mechanism by applying system biology-related tools to evaluate a data transcription database obtained form ArrayExpress (global database). Our hypothesis is that inflammation guides the process of differentiating mesenchymal stem cells into osteoblasts.