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Genomic instability in breast malignant tumors as related to the concentration of intracelular aluminium and to estrogen receptor status

Grant number: 08/02469-8
Support type:Regular Research Grants
Duration: February 01, 2009 - July 31, 2011
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Luís Otávio Zanatta Sarian
Grantee:Luís Otávio Zanatta Sarian
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


Introduction: This study protocol is related to other ongoing studies on the carcinogenesis of gynecological and breast malignancies. Evidence suggests that aluminium compounds may trigger mutagenic processes in breast tissues. These compounds can enter cells by food ingestion or dermic exposure to substances containing aluminium. After entering the tissues, aluminium compounds may mimic estrogen. High aluminium concentration and accumulation have been described in association to DNA alterations and genic abnormalities. There are no in vitro and in vivo studies examining the specific gene abnormalities caused by aluminium compounds in breast tumors. Objective: To determine the instability of genes C-MYC, CCND1 and ERBB2 in breast tumor tissues related to the presence of estrogen receptors and intracellular aluminium. Subjects and Methods: This will be a cross-sectional study, including samples from 150 subjects. The samples will be collected from women who underwent mastectomy, in the Gynaecological Oncology and Breast Pathology clinics of Unicamp. Tissue samples will be examined with routine pathological workup and fragments containing invasive carcinoma will be taken to determination of estrogen receptor, using immunohistochemistry, and for the quantification of intracellular aluminium with atomic absorption spectrophotometry. The genomic instability for the genes C-MYC, CCND1 and ERBB2 will be assessed with fluorescence in situ hybridization (FISH). Genomic instability of each gene will be examined with regards to the presence and quantity of intracellular aluminium. Significance levels of 5% will be used in statistical calculations (p<=0,05 and 95% CI). (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES-PERES, RAQUEL M.; CARVALHO, BENILTON S.; ANURAG, MEENAKSHI; LEI, JONATHAN T.; CONZ, LIVIA; GONCALVES, RODRIGO; CARDOSO FILHO, CASSIO; RAMALHO, SUSANA O. B.; DE PAIVA, GEISILENE R.; DERCHAIN, SOPHIE F. M.; LOPES-CENDES, ISCIA; ELLIS, MATTHEW J.; SARIAN, LUIS O. Z. Copy number alterations associated with clinical features in an underrepresented population with breast cancer. MOLECULAR GENETICS & GENOMIC MEDICINE, v. 7, n. 7 JUL 2019. Web of Science Citations: 0.
BAPTISTA, MAURICIO Z.; SARIAN, LUIS OTAVIO; DERCHAIN, SOPHIE F. M.; PINTO, GLAUCE A.; VASSAL, JOSE. Prognostic significance of PD-L1 and PD-L2 in breast cancer. HUMAN PATHOLOGY, v. 47, n. 1, p. 78-84, JAN 2016. Web of Science Citations: 82.
RODRIGUES-PERES, RAQUEL MARY; CADORE, SOLANGE; FEBRAIO, STEFANNY; HEINRICH, JULIANA KARINA; SERRA, KATIA PITON; DERCHAIN, SOPHIE F. M.; VASSALLO, JOSE; SARIAN, LUIS OTAVIO. Tissue Aluminum Concentration Does Not Affect the Genomic Stability of ERBB2, C-MYC, and CCND1 Genes in Breast Cancer. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 154, n. 3, p. 345-351, SEP 2013. Web of Science Citations: 3.
RODRIGUES-PERES, RAQUEL MARY; CADORE, SOLANGE; FEBRAIO, STEFANNY; HEINRICH, JULIANA KARINA; SERRA, KATIA PITON; DERCHAIN, SOPHIE F. M.; VASSALLO, JOSE; SARIAN, LUIS OTAVIO. Aluminum concentrations in central and peripheral areas of malignant breast lesions do not differ from those in normal breast tissues. BMC CANCER, v. 13, MAR 8 2013. Web of Science Citations: 9.
PALEARI, RENATA G.; PERES, RAQUEL M. R.; FLORENTINO, JULIANA O.; HEINRICH, JULIANA K.; BRAGANCA, WELBE O.; DEL VALLE, JULIO CESAR T.; ZEFERINO, LUIZ CARLOS; DERCHAIN, SOPHIE F. M.; SARIAN, LUIS OTAVIO. Reduced prevalence of the C825T polymorphism of the G-protein beta subunit gene in women with breast cancer. International Journal of Biological Markers, v. 26, n. 4, p. 234-240, OCT-DEC 2011. Web of Science Citations: 1.
RODRIGUES PERES, RAQUEL MARY; SERRA, KATIA PITON; DERCHAIN, SOPHIE F. M.; YOON, JUNG HYUN; PINTO, GLAUCE APARECIDA; ALVARENGA, MARCELO; SOARES, FERNANDO AUGUSTO; HEINRICH, JULIANA K.; DA CUNHA, ISABELA WERNECK; VASSALLO, JOSE; SARIAN, LUIS OTAVIO. Comparative evaluation of the erbB2 and hormone receptor status of neighboring invasive and in situ components of ductal carcinomas of the breast. International Journal of Biological Markers, v. 24, n. 4, p. 238-244, OCT-DEC 2009. Web of Science Citations: 1.

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