IL-1B, IL-6, IL-10 and TNF-a gene polymorphisms analyses in patients with TMJ internal derangements and osteoarthritis

• Grant number: 09/02520-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2009
• End date: December 31, 2011
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Celia Marisa Rizzatti Barbosa
• Grantee: Celia Marisa Rizzatti Barbosa
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Temporomandibular joint (TMJ) internal derangements (ID) and osteoarthritis (OA) are among the most common temporomandibular disorders (TMD). Inflammatory and anti-inflammatory cytokines are present in the TMJ synovial fluid (SF), however at higher concentrations in TMJ ID and OA patients. Besides, there are studies indicating that elevated levels of inflammatory cytokines, such as tumor necrosis factor alpha (TNF-a) and interleukins 1B and 6 (Il-1B and Il-6), in the SF of patients with chronic closed lock are poor clinical outcome predictors after TMJ irrigation treatment, while elevated levels of interleukin 10, an anti-inflammatory cytokine, predict treatment success. These information suggest that TMJ pathological conditions are related to an imbalance in cytokine production, so that the excess of inflammatory and/or the lack of anti-inflammatorymay favor the involvement of the joint. With regard to this imbalance, previously described polymorphisms in the genes that encode these molecules may respond for different production rates, so that it is possible to hypothesize that individuals carrying genotypes associated with augmented inflammatory cytokines and/or diminished anti-inflammatory production are at higher risk of TMJ ID and/or OA. Therefore, this study aims to investigate the occurrence of polymorphisms in TNF-a (G-308A), Il-1B (C-511T e C+3953T), Il-6 (C-174G) and Il-10 (C-592A) genes in healthy and TMJ ID and/or OA individuals, diagnosed according to the research diagnostic criteria for temporomandibular disorders (RDC/TMD), and identify if they can be considered risk markers for TMJ pathological conditions. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SMITH, SHAD B.; PARISIEN, MARC; BAIR, ERIC; BELFER, INNA; CHABOT-DORE, ANNE-JULIE; GRIS, PAVEL; KHOURY, SAMAR; TANSLEY, SHANNON; TOROSYAN, YELIZAVETA; ZAYKIN, DMITRI; et al. Genome-wide association reveals contribution of MRAS to painful temporomandibular disorder in males. Pain, v. 160, n. 3, p. 579-591, MAR 2019. (09/02520-6)

SANDERS, A. E.; JAIN, D.; SOFER, T.; KERR, K. F.; LAURIE, C. C.; SHAFFER, J. R.; MARAZITA, M. L.; KASTE, L. M.; SLADE, G. D.; FILLINGIM, R. B.; et al. GWAS Identifies New Loci for Painful Temporomandibular Disorder: Hispanic Community Health Study/Study of Latinos. JOURNAL OF DENTAL RESEARCH, v. 96, n. 3, p. 277-284, MAR 2017. (09/02520-6)