Vaccinal potential of virus inactivated by high hydrostatic pressure and epitope mapping with biotechnological impact

• Grant number: 08/09835-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2009
• End date: April 30, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Carlos Francisco Sampaio Bonafe
• Grantee: Carlos Francisco Sampaio Bonafe
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The project aims the developing and obtaining of inactivated viral preparations, with vaccinal potential for parvovirus, virus of interest for Brazilian swine production. For the viral inactivation it will be used high hydrostatic pressure in combination with other agents. Initially the efficiency of inactivation will be find in studies in vitro, and after in vivo, by determination of its immunogenicity. The epitopes with biotechnological interest will be identified by spot synthesis technique, which consists in planned array of peptides related to the target proteins. To evaluate the efficacy of the obtained preparations, swine SPF (specific pathogen free) will be first immunized with the respective inactivated virus, and then challenged with those not inactivated. The results will be compared using the vaccine commercially available. The project could generate patents of the inactivated preparations. The tobacco mosaic virus will be investigated as a model of antigenicity, since their preparation and study are very accessible. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, ANCELMO RABELO; YAMIN, MARRIAM; GAVA, DANIELLE; CIACCI ZANELLA, JANICE REIS; VICCARI GATTI, MARIA SILVIA; SAMPAIO BONAFE, CARLOS FRANCISCO; DE LIMA NETO, DANIEL FERREIRA. Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. VIROLOGY JOURNAL, v. 16, JUN 3 2019. (08/09835-0)

BISPO, JOSE A. C.; BONAFE, CARLOS F. S.; SANTANA, KLEYSE M. O. V.; SANTOS, ENNE C. A.. A comparison of drying kinetics based on the degree of hydration and moisture ratio. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 60, n. 1, p. 192-198, JAN 2015. (08/09835-0)