Natural history and risk factors associated with the incidence of Helicobacter pylori infection in Indian children under 5 years of ethnic Guarani

• Grant number: 10/51098-2
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2010
• End date: July 31, 2012
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Elisabete Kawakami
• Grantee: Elisabete Kawakami
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

It is known that childhood is the critical period for acquiring the infection by Helicobacter pylori, but it the exact mechanism of transmission is unknown. The most important risk factor for infection is the socioeconomic conditions experienced during childhood. Better understanding of the natural history of infection can help to prevent infection. For this, it becomes important to apply non-invasive diagnostic tests locally validated for detection of Helicobacter pylori. Objectives. 1. To evaluate the incidence and spontaneous elimination of Helicobacter pylori infection in children younger than 5 years, 2. To evaluate the risk factors associated with Helicobacter pylori infection in cases incidents. Methodology. Longitudinal cohort study. Methods. Study population: Guarani indigenous community. Inclusion criteria: all indigenous children without chronic illness, aged under 5 years of both sexes. Will be applied a clinical, demographic and epidemiological questionnaire, with weekly visits. Tests for H. pylori detection: breath test with 13C-urea (sensitivity 93.3%, 95% CI: 86.8% - 99.7%, specificity 96.2%, 95% CI: 93.6% - 98.8%) in children, families and people of greater contact in the village, and stool antigen test with monoclonal antibodies, sensitivity of 100%; 95% CI: 92.7 - 100%, specificity of 76.9%; 95% CI: 70,1 to 81.4%, increasing to 97.8% (95% CI: 94.7 to 99.0) with cutoff 0.400. The tests will be repeated every three months until the children complete 12 months of study entry. Infected patient: both tests positive, not infected: both tests negative. Statistical methods. Will be used logistic regression analysis and univariate analysis/multiple criterion with stepwise forward variable selection. Association test Chi-square of Pearson and trend will be used for binary response of interest and the variables of interest. The incidence rate and annual disposal will be calculated using the conversion and reversion of the results of tests for detection of H. pylori respectively and also will calculate the annual population rate of incidence and elimination of infection. Will be used the statistical software SPSS for Windows 12.0 and Stata 8.0 for Windows and the database will be done in Excel. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA ROQUE, JULIANA REJANE; MACHADO, RODRIGO STREHL; RODRIGUES, DOUGLAS; RECH, PATRICIA; KAWAKAM, ELISABETE. Prevalence of Helicobacter pylori infection in an indigenous community in São Paulo and associated factors: cross-sectional study. São Paulo Medical Journal, v. 135, n. 2, p. 140-145, MAR-APR 2017. (10/51098-2)