The Toll Like Receptors gene polymorphisms(TLR) involved in the inflammatory process of Helicobacter pylori and gastric carcinogenesis and assessment of changes in gene expression

• Grant number: 13/14022-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2013
• End date: October 31, 2015
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: Juliana Garcia de Oliveira Cucolo
• Grantee: Juliana Garcia de Oliveira Cucolo
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil
• Associated researchers: Ana Elizabete Silva ; Lucas Trevizani Rasmussen ; Spencer Luiz Marques Payão

Abstract

Gastric cancer represents a global health problem, with a neoplasm in which genetic and environmental factors are involved. H. pylori is a bacterium that colonizes the stomach and affects about 50% of the world population. The gastric infection by this microorganism has been strongly linked to the etiology of gastric carcinogenesis, since the active host inflammatory response, which may lead to chronic inflammation. Thus, it has been evaluated the association of genetic polymorphisms of factors involved in the inflammatory process which can modulate the pattern of immune response of the host. This is the case known as Toll like receptors (TLR) that recognize molecular patterns associated with the microorganisms, including TLR2 (bacterial lipoprotein receptor) and TLR9 (bacterial primers receiver) are involved in the response to infection by H. pylori. Given the scarcity of studies of polymorphisms of these genes in dyspeptic patients and its possible involvement in the pathogenesis of H. pylori and gastric carcinogenesis, we proposed to evaluate by PCR-RFLP TLR2 polymorphisms (rs3804099 and deletion -196 to -174) and TLR9 (rs5743836, rs187084) and in dyspeptic patients with gastric cancer and a group of individuals whose histopathology was considered Normal. In order to draw a profile of these genetic polymorphisms associated with increased risk for gastric lesions found and infection by H. pylori, as well as assess the expression levels in the gastric mucosa by PCR in real time and the possible association of these SNPs in the level of protein expression of the receptors. The molecular epidemiology studies and susceptibility to diseases are being intensified, so have been relevant to the understanding of individual variability and predisposition to cancer. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUSI, MANOELA DIAS; CAROLINE, DE MATOS LOURENCO; RASMUSSEN, LUCAS TREVIZANI; MARQUES PAYAO, SPENCER LUIS; TEIXEIRA ROSSI, ANA FLAVIA; SILVA, ANA ELIZABETE; DE OLIVEIRA-CUCOLO, JULIANA GARCIA. Toll-like receptor 9 polymorphisms and Helicobacter pylori influence gene expression and risk of gastric carcinogenesis in the Brazilian population. WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY, v. 11, n. 11, p. 998-1010, NOV 15 2019. (14/17716-1, 13/14022-6)

LOURENCO, CAROLINE DE MATOS; SUSI, MANOELA DIAS; ANTUNES DO NASCIMENTO, MARIAH CRISTINA; SERAFIM JUNIOR, VILSON; SIMEDAN VILA, ANA PAULA; RODRIGUES-FLEMMING, GABRIELA HELENA; GOLONI-BERTOLLO, ENY MARIA; SILVA, ANA ELIZABETE; DE OLIVEIRA-CUCOLO, JULIANA GARCIA. Characterization and strong risk association of TLR2 del -196 to -174 polymorphism and Helicobacter pylori and their influence on mRNA expression in gastric cancer. WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY, v. 12, n. 5, p. 535-548, MAY 15 2020. (13/14022-6)