Detection and description of germ cell markers in rats during embryonic phase: differences and similarities with mice

• Grant number: 09/07170-3
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2009
• End date: October 31, 2011
• Field of knowledge: Biological Sciences - Morphology - Embryology
• Principal Investigator: Taiza Stumpp Teixeira
• Grantee: Taiza Stumpp Teixeira
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Sertoli cell-germ cell interactions are fundamental for spermatogenesis. During embryonic gonad differentiation Sertoli cell-primordial germ cell (PGC) interactions are crucial for sex-differentiatiation. Any abnormality in these interactions may cause germ cell tumor in the adult male and sex reversal or hermaphroditism. Although it is known that Sertoli cell determine PGC sex, many controversies exist concerning PGC influence on Sertoli cell differentiation. Thus, the aim of this study is to investigate Sertoli cell-PGC interactions and relate them with the differentiation status of these cells. A morphological and molecular study will be performed. Sertoli cell-PGC interactions will be analyzed by transmission electron microscopy and immuno-labelling of specific proteins expressed by these cells. DNA and histone methylation of PGC and Sertoli cells will also be analyzed through 5-methylcitosine and H3K27me3 labelling. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ENCINAS, GISELLY; ZOGBI, CAMILA; STUMPP, TAIZA. Detection of Four Germ Cell Markers in Rats during Testis Morphogenesis: Differences and Similarities with Mice. Cells Tissues Organs, v. 195, n. 5, p. 443-455, 2012. (09/07170-3)