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Sanitary surveillance of bovine encephalitis: Neospora caninum as causative agent

Abstract

The Laboratory of anatomopathology of Instituto Biologico/SP is authorized by the Brazilian Ministry of Agriculture (MAPA) to perform the histopathological differential diagnosis of encephalitis and Bovine Spongiform Encephalopathy. From January 2004 to June 2007 were examined by histopathology 690 brains from cattle with neurological syndrome negative to rabies (direct immunofluorescence), 40.0% (276) presented lesions, and the non purulent meningoencephalitis was the most frequent - 90.0% (249/276). This inflammatory infiltrate characterized by mononuclear cells indicates that viruses, parasites or bacterias are causing encephalitis, and that is necessary to use specific and sensitive tests to diagnose then. The present study proposes to diagnose N. caninum in bovine brain samples sent to Instituto Biológico perform the differential diagnosis of neurological syndrome, by the association of lesions by histology (Hematoxilin/Eosin stainning), the specificity of IHC and the specificity and sensitivity of two protocols for nested-PCR (genomic and ribosomal DNA) in cattle negative for rabies. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALBINATI MALAGUTI, JANE MARY; CABRAL, ALINE DINIZ; ABDALLA, RAISA PEREIRA; SALGUEIRO, YOLANDA OLIVEIRA; CACCIATORI GALLETI, NARA THIERS; OKUDA, LIRIA HIROMI; SEQUETIN CUNHA, ELENICE MARIA; PITUCO, EDVIGES MARISTELA; DEL FAVA, CLAUDIA. Neospora caninum as causative agent of bovine encephalitis in Brazil. Revista Brasileira de Parasitologia Veterinária, v. 21, n. 1, p. 48-54, JAN-MAR 2012. Web of Science Citations: 4.

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