Gold nano-probe for Paracoccidioides brasiliensis diagnoses

• Grant number: 09/09559-5
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2009
• End date: January 31, 2012
• Field of knowledge: Health Sciences - Collective Health - Epidemiology
• Principal Investigator: Leandro José Raniero
• Grantee: Leandro José Raniero
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

The main of this project is the development of an effective method to identify the Paracoccidioides Brasiliensis fungus, which causes paracoccidioidomycosis. This disease affects mainly the lungs and may be spread by hematogenic via. The most difficult part of treating this disease is the correct diagnosis, because there is a variety of fungi types and also the symptoms similarity with other diseases, such as those produced by tuberculosis. In fact, the correct identification of a particular fungus type is still difficult and expensive process, even with the recent equipments. In this context, nanotechnology brings new forms of analysis, quick and accurate in the identification of microorganisms by means of a specific gene sequence combined with metallic nanoparticles. This technology which is more current in terms of diseases diagnosis and, it is a method to identify the presence of a gene or protein fragment, with precision and a minimal amount of sample. Gold nanoparticles are functionalized with a specific genetic sequence of the Paracoccidioides Brasiliensis fungus, building a DNA nanoprobe. The fungus DNA will be extracted and amplified, and then will be used to identify the presence of the fungus by optical spectroscopy. The domain of correct fungus identification will lead the application of this analysis in body fluids that may be contaminated with microorganism. The development of the project can move to build a diagnostic system using the Raman Effect and this biosensor. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTILHO, M. L.; VIEIRA, L. S.; CAMPOS, A. P. C.; ACHETE, C. A.; CARDOSO, M. A. G.; RANIERO, L.. The efficiency analysis of gold nanoprobes by FT-IR spectroscopy applied to the non-cross-linking colorimetric detection of Paracoccidioides brasiliensis. SENSORS AND ACTUATORS B-CHEMICAL, v. 215, p. 258-265, AUG 2015. (13/17404-7, 09/09559-5)

COMPARATO FILHO, OLAVO O.; MORAIS, V, FLAVIA; BHATTACHARJEE, TANMOY; CASTILHO, MAIARA L.; RANIERO, LEANDRO. Rapid identification of Paracoccidioides lutzii and P-Brasiliensis using Fourier Transform Infrared spectroscopy. Journal of Molecular Structure, v. 1177, p. 152-159, FEB 5 2019. (13/17404-7, 09/09559-5)

MARTINS, JACIARA F. S.; CASTILHO, MAIARA L.; CARDOSO, MARIA A. G.; CARREIRO, ANDREA P.; MARTIN, AIRTON A.; RANIERO, LEANDRO; MAHADEVANJANSEN, A; PETRICH, W. Identification of Paracoccidioides brasiliensis by Gold Nanoprobes. BIOMEDICAL VIBRATIONAL SPECTROSCOPY V: ADVANCES IN RESEARCH AND INDUSTRY, v. 8219, p. 6-pg., 2012-01-01. (09/09559-5)

FERREIRA, ISABELLE; FERREIRA-STRIXINO, JULIANA; CASTILHO, MAIARA L.; CAMPOS, CLAUDIA B. L.; TELLEZ, CLAUDIO; RANIERO, LEANDRO. Characterization of Paracoccidioides brasiliensis by FT-IR spectroscopy and nanotechnology. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 152, p. 397-403, JAN 5 2016. (09/09559-5)

FAGUNDES, JACIARA; CASTILHO, MAIARA L.; TELLEZ SOTO, CLAUDIO A.; VIEIRA, LAIS DE SOUZA; CANEVARI, RENATA A.; FAVERO, PRISCILA P.; MARTIN, AIRTON A.; RANIERO, LEANDRO. Ribosomal DNA Nanoprobes studied by Fourier Transform Infrared spectroscopy. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 118, p. 28-35, JAN 24 2014. (09/09559-5)

CASTILHO, MAIARA L.; CAMPOS, CLAUDIA B. L.; MATOS, TATIANA G. F.; DE ABREU, GERALDO M. A.; MARTIN, AIRTON A.; RANIERO, LEANDRO; MAHADEVANJANSEN, A; PETRICH, W. Biochemical differentiation of mycelium and yeast forms of Paracoccidioides brasiliensis by Fourier Transform Infrared Spectroscopy. BIOMEDICAL VIBRATIONAL SPECTROSCOPY V: ADVANCES IN RESEARCH AND INDUSTRY, v. 8219, p. 6-pg., 2012-01-01. (09/09559-5)

AGUIAR, J. C.; MITTMANN, J.; CAETANO JUNIOR, P. C.; RANIERO, L.. Using FT-IR spectroscopy for the identification of the T-cruzi, T-rangeli, and the L-chagasi species. Experimental Parasitology, v. 192, p. 46-51, SEP 2018. (09/09559-5, 13/17404-7)