Genetic variants envolved in folate metabolism: impact on head and neck carcinogenesis

• Grant number: 10/00012-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2010
• End date: June 30, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Eny Maria Goloni Bertollo
• Grantee: Eny Maria Goloni Bertollo
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Changes in folate metabolism may contribute to the process of carcinogenesis by influencing the reactions of DNA methylation and genomic stability. Polymorphisms in genes encoding enzymes involved in the folate pathway may alter enzyme activity and consequently interfere in concentrations of homocysteine, S-adenosylmethionine and other products of metabolism which are important for DNA synthesis and cellular methylation reactions. The objective of this project is to investigate polymorphisms of BHMT, DHFR, SHMT, MTHFD1 and TC2 genes, which participate in the metabolic pathway of folate, in the risk for head and neck cancer and also investigate the association between these polymorphisms and the clinical characteristics of patients. The genetic changes will be investigated by the techniques of Polymerase Chain Reaction (PCR), Real-Time PCR and analysis of Restriction Fragment Length Polymorphism (PCR-RFLP). The results of this project will contribute to understanding the involvement of genes from the folate pathway in the development of squamous cell carcinoma of head and neck and to clarify the mechanisms leading to the tumorigenesis process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUCCI, MAYSA; DE CASTRO, TIALFI BERGAMIN; SILVA GALBIATTI, ANA LIVIA; REBOLHO BATISTA ARANTES, LIDIA MARIA; GOMES DA SILVA, JESSIKA NUNES; MANIGLIA, JOSE VICTOR; RAPOSO, LUIZ SERGIO; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. DNMT3B C46359T and SHMT1 C1420T polymorphisms in the folate pathway in carcinogenesis of head and neck. MOLECULAR BIOLOGY REPORTS, v. 41, n. 2, p. 581-589, FEB 2014. (10/00012-0)

SILVA GALBIATTI, ANA LIVIA; RUIZ, MARIANGELA TORREGLOSA; PINTO, DANIELA REZENDE; RAPOSO, LUIZ SERGIO; MANIGLIA, JOSE VICTOR; PAVARINO-BERTELLI, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. A80G polymorphism of reduced folate carrier 1 (RFC1) gene and head and neck squamous cell carcinoma etiology in Brazilian population. MOLECULAR BIOLOGY REPORTS, v. 38, n. 2, p. 1071-1078, FEB 2011. (10/00012-0)

SILVA GALBIATTI, ANA LIVIA; REBOLHO BATISTA DA SILVA, LIDIA MARIA; RUIZ-CINTRA, MARIANGELA TORREGLOSA; RAPOSO, LUIS SERGIO; MANIGLIA, JOSE VICTOR; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. Association between 11 genetic polymorphisms in folate-metabolising genes and head and neck cancer risk. EUROPEAN JOURNAL OF CANCER, v. 48, n. 10, p. 1525-1531, JUL 2012. (10/00012-0)