Effects of thymulin 5cH in BCG-induced granuloma: the B-1 cell participation

• Grant number: 10/50842-0
• Support type: Regular Research Grants
• Duration: October 01, 2010 - September 30, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Leoni Villano Bonamin
• Grantee: Leoni Villano Bonamin
• Home Institution: Vice-Reitoria de Pós-Graduação e Pesquisa. Universidade Paulista (UNIP). São Paulo , SP, Brazil

Abstract

In previous studies, it was observed that thymulin prepared homeopathically (5cH potency) is able to improve broiler production, as well as to modulate the Ehrlich tumor development and BCG induced-granuloma in mice, involving immune modulation mechanisms. Herein, a deeper approach about these modulation mechanisms is proposed, with special focus on the leukocyte migration to the lesion, mainly B-1 cells. Male Balb/c mice will be inoculated with BCG, treated orally with thymulin 5cH or vehicle, and the subcutaneous granuloma and the local lymph node will be studied, regarding to the phenotypical characterization of inflammatory cells using immunohistochemistry and flow cytometry techniques. In the first case, the number and distribution of positive cells for CD3, CD11b, CD54R, pan-cytokeratin (epithelioid cells) will be evaluated. In the second case, the quantification of positive cells for CD11b, CD19, CD23, CD5, CD25 (regulatory T cells), CD4, CD8 and CD11c (dendritic cells) will be done. The statistical analysis will be Student "t" test or X2, being p=0,05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SANTANA, FABIANA RODRIGUES; COELHO, CIDELI DE PAULA; CARDOSO, THAYNA NEVES; LAURENTI, MARCIA DALASTRA; PEREZ HURTADO, ELIZABETH CRISTINA; BONAMIN, LEONI VILLANO. Modulation of inflammation response to murine cutaneous Leishmaniosis by homeopathic medicines: Thymulin 5cH. Homeopathy, v. 103, n. 4, p. 275-284, OCT 2014. Web of Science Citations: 9.

DE SANTANA, FABIANA RODRIGUES; COELHO, CIDELI DE PAULA; CARDOSO, THAYNA NEVES; PEREZ HURTADO, ELIZABETH CRISTINA; BENITES, NILSON ROBERTI; LAURENTI, MARCIA DALASTRA; BONAMIN, LEONI VILLANO. Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH. Homeopathy, v. 103, n. 4, p. 264-274, OCT 2014. Web of Science Citations: 8.

BONAMIN, LEONI VILLANO; SATO, CESAR; NETO, RUGGERO ZALLA; MORANTE, GRAZIELA; CARDOSO, THAYNA NEVES; DE SANTANA, FABIANA RODRIGUES; COELHO, CIDELI DE PAULA; OSUGUI, LIKA; POPI, ANA FLAVIA; PEREZ HURTADO, ELIZABETH CRISTINA; MARIANO, MARIO. Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model. Evidence-based Complementary and Alternative Medicine, 2013. Web of Science Citations: 5.