Biotransformation of natural and synthetic products using endophytic microorganisms, a chemical-ecologycal approach for the production of bioactive substances

• Grant number: 10/11384-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2010
• End date: April 30, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Edson Rodrigues Filho
• Grantee: Edson Rodrigues Filho
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The current and urgent concerns with the protection and preservation of the environment have stimulated the chemists involved with production processes of intermediary compounds and end-products for the consumption of the population to develop and to use more and more efficient and less polluting methodologies. The use of enzyme comes being pointed as an excellent possible strategy for reduction of solvent and steps that uses toxic metallic catalysts, to maximize the yield of chemical conversions and to get good stereochemical control in multi-steps syntheses. Enzymes can be gotten from diverse animal and vegetal sources. However the microorganisms are promising sources due to its small cycle of life and to the easiness of manipulation and maintenance. Fungi, yeasts and bacteria have been submitted to intense programs of selections, many times randomly, to find useful enzyme. We present here a different approach for the discovery of enzymatic chemical processes, studying the ecology and the biochemical adaptation that endophytic microorganisms undergoes during numerous life cycles to associate with the plants that host them. This particular developed biochemical association, together with the study of the secondary metabolism of these microorganisms, comes serving as a good guide for the detection of useful enzyme for transformation of natural products isolated from host plants and its structural analogous obtained synthetically, to generate bioactive substances. In this project, it is intended to characterize and isolate enzymes aiming to a large scale production. The bioactivity assays will include cytotoxycity activity, studies of mechanisms of cellular inhibition, besides antimicrobial and insecticidal activity. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FILL, TAICIA PACHECO; BARETTA, JESSICA FERNANDA; PAULA DE ALMEIDA, LUIZ GONZAGA; MALAVAZI, IRAN; CERDEIRA, LOUISE TEIXEIRA; SAMBORSKYY, MARKIYAN; RIBEIRO DE VASCONCELOS, ANA TEREZA; LEADLAY, PETER; RODRIGUES-FILHO, EDSON. Draft Genome Sequence of the Fungus Penicillium brasilianum (Strain LaBioMMi 136), a Plant Endophyte from Melia azedarach. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 7, n. 21, NOV 2018. (10/11384-6, 15/10384-6)

DIN, ZIA UD; FILL, TAICIA P.; CAROLINA DONATONI, M.; DOS SANTOS, CAROLINA A. A.; BROCKSOM, TIMOTHY J.; RODRIGUES-FILHO, E.. Microbial diversification of Diels-Alder cycloadducts by whole cells of Penicillium brasilianum. MOLECULAR DIVERSITY, v. 20, n. 4, p. 877-885, NOV 2016. (10/11384-6, 11/13993-2)

AMARAL, LUCIANA DA SILVA; RODRIGUES-FILHO, EDSON. Aryl carboxylic acid reduction and further reactions with GABA and glucose promoted by whole cells of Xylaria arbuscula. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v. 113, p. 90-94, MAR 2015. (10/11384-6, 13/18603-3)

FILL, TAICIA PACHECO; PALLINI, HELOISA FASSINA; DIN, ZIA UD; JURBERG, IGOR DIAS; DA SILVA, JOSE VINICIUS; RODRIGUES-FILHO, EDSON. Conjugation of antifungal benzoic acid derivatives as a path for detoxification in Penicillium brasilianum, an endophyte from Melia azedarach. BIOORGANIC CHEMISTRY, v. 81, p. 367-372, DEC 2018. (10/11384-6, 15/10384-6)

DIN, ZIA UD; TRAPP, MARILIA ALMEIDA; DE MEDEIROS, LIVIA SOMAN; LAZARIN-BIDOIA, DANIELLE; GARCIA, FRANCIELLE PELEGRIN; PERON, FRANCIELI; NAKAMURA, CELSO VATARU; RODRIGUEZ, IHOSVANY CAMPS; WADOOD, ABDUL; RODRIGUES-FILHO, EDSON. Symmetrical and unsymmetrical substituted 2,5-diarylidene cyclohexanones as anti-parasitic compounds. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, v. 155, p. 596-608, JUL 15 2018. (10/11384-6)

DIN, ZIA UD; LAZARIN-BIDOIA, DANIELLE; KAPLUM, VANESSA; GARCIA, FRANCIELLE PELEGRIN; NAKAMURA, CELSO VATARU; RODRIGUES-FILHO, EDSON. The structure design of biotransformed unsymmetrical nitro-contained 1,5-diaryl-3-oxo-1, 4-pentadienyls for the anti-parasitic activities. ARABIAN JOURNAL OF CHEMISTRY, v. 12, n. 8, p. 4006-4016, DEC 2019. (10/11384-6)

DIN, ZIA UD; RODRIGUES-FILHO, EDSON. Optimized one-pot synthesis of monoarylidene and unsymmetrical diarylidene cycloalkanones. ARABIAN JOURNAL OF CHEMISTRY, v. 12, n. 8, p. 4756-4763, DEC 2019. (10/11384-6)

DIN, ZIA UD; DOS SANTOS, ALEF; TRAPP, MARILIA ALMEIDA; LAZARIN-BIDOIA, DANIELLE; GARCIA, FRANCIELLE PELEGRIN; PERON, FRANCIELI; NAKAMURA, CELSO VATARU; RODRIGUES-FILHO, EDSON. Curcumin inspired synthesis of unsymmetrical diarylpentanoids with highly potent anti-parasitic activities: in silico studies and DFT-based stereochemical calculation. MedChemComm, v. 7, n. 5, p. 820-831, 2016. (10/11384-6)