Schistosoma genome

• Grant number: 01/04349-0
• Support Opportunities: Genome Research Grants
• Start date: June 01, 2001
• End date: February 29, 2004
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Cybele Gargioni
• Grantee: Cybele Gargioni
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The aim of this project is to sequence in the next 18 months 120,000 EST clones generated by the ORESTES low-stringency RT-PCR amplification technique (Dias-Neto et al., PNAS USA. 97: 3491-3496, 2000) using S. mansoni mRNA. We expect to generate 40 to 50 million bases of S. mansoni cDNA, which might represent an estimated average 1 to 1.5 fold coverage of the parasite transcriptome. Different stages in the life cycle of the parasite will be studied. We expect to collect approximately 15 to 20 thousand ESTs from each different stage of the cycle including cercariae, miracidium, adult worms (male/female), eggs and 7-day circulating schistosomula. (AU)