Establishment of conditions for the disruption genes potentially involved in Xylella fastidiosa pathogenesis

• Grant number: 98/16251-0
• Support Opportunities: Genome Research Grants
• Start date: March 01, 1999
• End date: June 30, 2003
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Suely Lopes Gomes
• Grantee: Suely Lopes Gomes
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The purpose of this project is to establish conditions for gene disruption in Xylella fastidiosa, aiming at genes potentially involved in bacterial pathogenesis. Two main strategies will be attempted: (a) electroporation of Xylella fastidiosa with a suicide vector containing the gene of interest interrupted with a cassette encoding an antibiotic resistance gene; (b) conjugation of Xylella fastidiosa with an Escherichia coli donor strain carrying the suicide vector described in (a). Once the Xylella fastidiosa strains carrying a disruption in each of the target genes have been obtained, pathogenicity tests will have to be carried out. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA NETO‚ J.F.; KOIDE‚ T.; GOMES‚ S.L.; MARQUES‚ M.V.. Site-directed gene disruption in Xylella fastidiosa. FEMS Microbiology Letters, v. 210, n. 1, p. 105-110, 2002. (98/16251-0)

KOIDE, TIE; SILVA NETO, JOSÉ F. DA; GOMES, SUELY LOPES; MARQUES, MARILIS V.. Insertional transposon mutagenesis in the Xylella fastidiosa citrus variegated chlorosis strain with transposome. Current Microbiology, v. 48, n. 4, p. 247-250, Apr. 2004. (98/16251-0)