Optimization of cultivation conditions of yeasts isolated from Brazilian biomes to obtain SCP (single cell protein) using xylose and bagasse hydrolysates of sugar cane as raw material

• Grant number: 10/51507-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2010
• End date: October 31, 2013
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Maria da Graça Stupiello Andrietta
• Grantee: Maria da Graça Stupiello Andrietta
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil
• Associated scholarship(s): 11/15970-0 - Optimization of cultivation conditions of yeasts isolated from Brazilian biomes to obtain SCP (single cell protein) using xylose and bagasse hydrolysates of sugar cane as raw material, BP.TT

Abstract

The purpose of this study is evaluate xylose use by yeasts as carbon source for obtaining SCP. The yeasts involved in this study were isolated from Brazilian biomes (Cerrado and the Atlantic Forest). This study includes an initial screening, where nineteen yeasts will be grown in synthetic medium containing xylose as carbon source. From the results three strains will be chosen, taking into account the amount of protein obtained from cell mass. An optimization study using the experimental design will be developed for the three yeasts. The dependent variables of this optimization are the following parameters: (a) temperature, (b) agitation (rpm) and (c) concentration of xylose in the culture medium. The response variable is the amount of protein obtained from cell mass. The specific growth rate (p max) for the yeasts chosen will also be obtained in this study. The project also evaluates the performance of yeast in relation to the levels of cellular protein when grown in industrial culture medium (bagasse hydrolyzate). Yeasts will be identified using molecular biology techniques. (AU)