| Grant number: | 99/05818-2 |
| Support type: | BIOTA-FAPESP Program - Regular Research Grants |
| Duration: | January 01, 2000 - March 31, 2002 |
| Field of knowledge: | Biological Sciences - Genetics - Plant Genetics |
| Principal Investigator: | Maria Teresa Vitral de Carvalho Derbyshire |
| Grantee: | Maria Teresa Vitral de Carvalho Derbyshire |
| Home Institution: | Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil |
Abstract
The present project aims to study the genetic structure of natural populations of Cryptocarya aschersoniana Mez, C. moschata Nees, C. saligna Mez and Cryptocarya spp. (new species) through isoenzymatic and DNA (RAPD) markers, based on a sampling to be undertaken in the Carlos Botelho State Park (on an altitudinal gradient of 30 to 1000 m), the Juréia-Itatins Ecological Station, the Experimental Station of Pariqüera-Açu, the Cantareira State Park, Forest of Santa Genebra, Barreiro Rico Farm, Campininha Farm, São José Farm, Santa Elisa (IAC) Farm and Bosque dos Jequitibás, State of São Paulo. Based on this basic study, we will investigate several aspects of the life history of these species, such as genetic flux and effective population size, which will be used to elaborate strategies for sampling, management and conservation of the same. Additionally, we will analyze the materials deposited in Brazilian herbariums, together with the samples collected, so as to establish a methodology for the study of the phylogeny of these species, through PCR of genes of chloroplast and mitochondria and sequencing of specific genes, with the aim of solving several taxonomic questions found in this group of plants, as yet unresolved given merely the base of classical morphological characters. (AU)