Evaluation of VEGF, VEGFR and matrix metalloproteinase-9 immunohistochemical expressions as potential prognostic indicators for canine cutaneous mast cell tumors

• Grant number: 10/05094-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2010
• End date: May 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Ricardo de Francisco Strefezzi
• Grantee: Ricardo de Francisco Strefezzi
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Heidge Fukumasu ; José Guilherme Xavier ; Jose Luiz Catao Dias ; Maria Cecília Rui Luvizotto ; Silvia Regina kleeb

Abstract

Mast cell tumors are frequent neoplasms and usually regarded as potentially malignant, thus representing prognostic and therapeutic challenges for veterinarians. Several researches have been done in search for more precise biological behavior indicators for this tumor. Angiogenesis and extracellular matrix degradation are important steps to invasion of adjacent tissues and neoplastic dissemination. These phenomena contribute to malignancy and are regulated by many growth factors and proteolytic enzymes, currently under research. The objective of this study is to characterize the immunohistochemical expressions of the Vascular Endothelial Growth Factor and its receptor (VEGF and VEGFR), and of the Matrix Metalloproteinase 9 (MMP-9) in canine cutaneous mast cell tumors, in comparison to the histomorphological grading system and the clinical evolution of the cases. The mast cell tumors will be submitted to immunohistochemical analysis, followed by quantification of the reactions by computerized morphometry. The results will be compared to the histopathological grading and to the survival and/or time to recurrence for the validation of these markers as prognostic indicators for this neoplasm. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANIEL, JESSIKA; BARRA, CAMILA N.; PULZ, LIDIA H.; KLEEB, SILVIA REGINA; XAVIER, JOSE GUILHERME; CATAO-DIAS, JOSE LUIZ; NISHIYA, ADRIANA T.; FUKUMASU, HEIDGE; STREFEZZI, RICARDO F.. Intratumoral collagen index predicts mortality and survival in canine cutaneous mast cell tumours. VETERINARY DERMATOLOGY, v. 30, n. 2, p. 162+, APR 2019. (13/13252-8, 16/03862-1, 10/05094-5)

VARGAS, T. H. M.; PULZ, L. H.; BARRA, C. N.; KLEEB, S. R.; XAVIER, J. G.; CATAO-DIAS, J. L.; FUKUMASU, H.; NISHIYA, A. T.; STREFEZZI, R. F.. Immunohistochemical Expression of the Pluripotency Factor OCT4 in Canine Mast Cell Tumours. Journal of Comparative Pathology, v. 153, n. 4, p. 251-255, NOV 2015. (10/05094-5, 13/13252-8)

PULZ, L. H.; BARRA, C. N.; KLEEB, S. R.; XAVIER, J. G.; CATAO-DIAS, J. L.; SOBRAL, R. A.; FUKUMASU, H.; STREFEZZI, R. F.. Increased expression of tissue inhibitor of metalloproteinase-1 correlates with improved outcome in canine cutaneous mast cell tumours. VETERINARY AND COMPARATIVE ONCOLOGY, v. 15, n. 2, p. 606-614, JUN 2017. (10/05094-5, 13/13252-8)

PULZ, L. H.; STREFEZZI, R. F.. Proteases as prognostic markers in human and canine cancers. VETERINARY AND COMPARATIVE ONCOLOGY, v. 15, n. 3, p. 669-683, SEP 2017. (10/05094-5, 13/13252-8)