Systemic analysis, metabolic engineering and economic evaluation of hemicellulosic hydrolysate utilization in bioprocesses

• Grant number: 10/51989-4
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: January 01, 2011
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luiziana Ferreira da Silva
• Grantee: Luiziana Ferreira da Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project is related with the production of biofuels and bioplastics from lignocellulosic residues. Among the sugars of lignocellulose, the xylose, present in the hemicellulose, is recovered more easily and in better yields than glucose. The objective of the work is to increase the efficiency (yield and productivity) of bioprocesses using the biomass hemicellulosic fraction. Strains tolerant to the inhibitors present in the hemicellulosic hydrolysates will be used: the ethalonogenic Escherichia coli KO11 and the PHA-producing Burkholderia sp. F24. This research focuses on fundamental understanding of those cellular factors that we consider still the most essential in improvement of xylose utilization by bacteria and of process consolidation aspects to reach the level required in commercial production of biofuels and bioplastics from the hemicellulosic hydrolysates. The following strategies are proposed: (i) construction of genome-based in silico models of xylose metabolism, (ii) through analysis of those in silico models it will be proposed a metabolic engineering strategy to increase yield and productivity of the bioproduct. (iii) Ecobiotechnology techniques will also be used, such as evolutionary engineering and aerobic dynamic feeding, to obtain strains with better performance (in collaboration with Dr. M. Reis, University of Nova Lisboa), (iv) The best strains will be used in to high cell density fed-bacth cultivations using hemicellulosic hydrolysate as carbon source. In some of those steps, hydrolysates could be produced in cooperation with Dr. A. Aprigio, Physics-Chemistry Institute, USP, São Carlos.(v) From yield and productivity values obtained in this project, a preliminary economic evaluation will be performed to verify the economic potential of this technology. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA-SILVA, LUIZIANA; TACIRO, MARILDA KEICO; RAICHER, GIL; MONIZ PICCOLI, ROSANE APARECIDA; MENDONCA, THATIANE TEIXEIRA; GARCEZ LOPES, MATEUS SCHREINER; CABRERA GOMEZ, JOSE GREGORIO. Perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. International Journal of Biological Macromolecules, v. 71, n. SI, p. 2-7, NOV 2014. (10/51989-4, 10/51692-1)

RADUAN ALEXANDRINO, PAULO MOISES; MENDONCA, THATIANE TEIXEIRA; GUAMAN BAUTISTA, LINDA PRISCILA; CHERIX, JULIANO; LOZANO-SAKALAUSKAS, GABRIELA CAZONATO; FUJITA, ANDRE; RAMOS FILHO, EDMAR; LONG, PAUL; PADILLA, GABRIEL; TACIRO, MARILDA KEICO; et al. Draft Genome Sequence of the Polyhydroxyalkanoate-Producing Bacterium Burkholderia sacchari LMG 19450 Isolated from Brazilian Sugarcane Plantation Soil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3, MAY-JUN 2015. (10/51989-4, 12/51533-6)

GUAMAN, LINDA P.; OLIVEIRA-FILHO, EDMAR R.; BARBA-OSTRIA, CARLOS; GOMEZ, JOSE G. C.; TACIRO, MARILDA K.; DA SILVA, LUIZIANA FERREIRA. xylA and xylB overexpression as a successful strategy for improving xylose utilization and poly-3-hydroxybutyrate production in Burkholderia sacchari. Journal of Industrial Microbiology & Biotechnology, v. 45, n. 3, p. 165-173, MAR 2018. (10/51989-4, 16/00842-0)

FERREIRA-SILVA, LUIZIANA; TACIRO, MARILDA KEICO; RAICHER, GIL; MONIZ PICCOLI, ROSANE APARECIDA; MENDONCA, THATIANE TEIXEIRA; GARCEZ LOPES, MATEUS SCHREINER; CABRERA GOMEZ, JOSE GREGORIO. Perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. International Journal of Biological Macromolecules, v. 71, p. 6-pg., 2014-11-01. (10/51692-1, 10/51989-4)

GUAMAN, LINDA P.; BARBA-OSTRIA, CARLOS; ZHANG, FUZHONG; OLIVEIRA-FILHO, EDMAR R.; GOMEZ, JOSE GREGBRIO C.; SILVA, LUIZIANA F.. Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari. Microbial Cell Factories, v. 17, MAY 15 2018. (10/51989-4, 16/00842-0)

RADUAN ALEXANDRINO, PAULO MOISES; MENDONCA, THATIANE TEIXEIRA; GUAMAN BAUTISTA, LINDA PRISCILA; CHERIX, JULIANO; LOZANO-SAKALAUSKAS, GABRIELA CAZONATO; FUJITA, ANDRE; RAMOS FILHO, EDMAR; LONG, PAUL; PADILLA, GABRIEL; TACIRO, MARILDA KEICO; et al. Draft Genome Sequence of the Polyhydroxyalkanoate-Producing Bacterium Burkholderia sacchari LMG 19450 Isolated from Brazilian Sugarcane Plantation Soil. GENOME ANNOUNCEMENTS, v. 3, n. 3, p. 2-pg., 2015-05-01. (12/51533-6, 10/51989-4)