Infection by Giardia lamblia: search for molecules involved in parasita-host interaction

• Grant number: 10/15042-2
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: April 01, 2011
• End date: March 31, 2015
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Renata Rosito Tonelli
• Grantee: Renata Rosito Tonelli
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated scholarship(s): 13/04272-5 - The role of SUMOylation of proteins in cell biology of Giardia lamblia and in the interaction with the host, BP.DD; 11/14573-7 - Study of post-translational modification on claudins, a family of TJ proteins, on the establishment of giardiasis, BP.MS

Abstract

Giardia is the most prevalent human intestinal parasitic protist in the world, and is a major cause of diarrhea in children and travelers. Infection causes diarrhea via a combination of intestinal malabsorption and hypersecretion as a result from disruption of the epithelial tight junctions. A pre-requisite for the establishment of infection involves the colonization of the small intestine with Giardia trophozoites which adhere to the intestinal epithelia to avoid being expelled by the intestinal fluids. The host-parasite interactions that govern the outburst of infection remain largely obscure. The aim of this project is to study the mechanisms involved in the loss of the intestinal epithelial barrier and to identify the parasite molecules possibly related to these events upon infection with Giardia, as well as to identify the host cell receptors for these molecules and to study the events that leads to the loss of the epithelial barrier. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DI GENOVA, BRUNO M.; DA SILVA, RICHARD C.; DA CUNHA, JULIA P. C.; GARGANTINI, PABLO R.; MORTARA, RENATO A.; TONELLI, RENATA R.. Protein SUMOylation is Involved in Cell-cycle Progression and Cell Morphology in Giardia lamblia. Journal of Eukaryotic Microbiology, v. 64, n. 4, p. 491-503, JUL-AUG 2017. (10/15042-2, 13/04272-5)

BARTOLONI, FERNANDO H.; GONCALVES, LETICIA CHRISTINA P.; RODRIGUES, ANA CLARA B.; DOERR, FELIPE AUGUSTO; PINTO, ERNANI; BASTOS, ERICK L.. Photophysics and hydrolytic stability of betalains in aqueous trifluoroethanol. MONATSHEFTE FUR CHEMIE, v. 144, n. 4, p. 567-571, APR 2013. (07/00684-6, 07/59407-1, 10/15042-2, 10/16082-8, 11/23036-5)

TONELLI, R. R.; COLLI, W.; ALVES, M. J. M.. Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro. FRONTIERS IN IMMUNOLOGY, v. 3, 2013. (10/15042-2)

DI GENOVA, BRUNO M.; TONELLI, RENATA R.. Infection Strategies of Intestinal Parasite Pathogens and Host Cell Responses. FRONTIERS IN MICROBIOLOGY, v. 7, MAR 3 2016. (10/15042-2, 13/04272-5)

PIRES GONCALVES, LETICIA CHRISTINA; TONELLI, RENATA ROSITO; BAGNARESI, PIERO; MORTARA, RENATO ARRUDA; FERREIRA, ANTONIO GILBERTO; BASTOS, ERICK LEITE. A Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytes. PLoS One, v. 8, n. 1, JAN 16 2013. (11/23036-5, 07/00684-6, 07/59407-1, 10/15042-2)