Acquisition of an ultracentrifuge and an echocardiograph to equip a Centralized Multi-User Laboratory

• Grant number: 09/54102-3
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2010
• End date: September 30, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Marina Politi Okoshi
• Grantee: Marina Politi Okoshi
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The project aims to equip a multi-user centralized laboratory at the Botucatu School of Medicine (FMB), designated the Unipex (Experimental Research Unit). The Unipex is an experimental research laboratory and research unit for animal experiments, funded with support from the Ministry of Science and Technology and the Financing Agency for Studies and Projects (grant n. 0112007 and 0112008) and through partnerships with the São Paulo State University and the FMB Foundation for Physician and Hospital Development. The researchers have extensive experience in the study of cardiac remodeling and its systemic consequences in different experimental models of heart injury. The analysis of cardiac structures and ventricular function is typically assessed in vivo by echocardiography. Because echocardiography is noninvasive, it is possible to perform longitudinal studies of serial evaluations of the heart, with animals under light anesthesia. Currently, echocardiographic examinations in animal experiments are conducted on equipment located in the FMB Clinics Hospital. However, that equipment, which is used for clinical and experimental research by investigators at the FMB and at the Botucatu Institute of Biosciences, has reached the end of its useful life and relevance as a technological resource. We therefore request a new echocardiograph to be included within the Unipex equipment park. The study of the mechanisms involved in cardiac remodeling and their systemic consequences involves the evaluation of intracellular signaling pathways. For analysis of intracellular signaling in structures such as nuclei, mitochondria and membranes, there is a need for ultracentrifugation in order to separate and obtain intracellular components. As the FMB has no ultracentrifuge, its members will also benefit from the acquisition of this equipment for the Unipex, where it will be available to researchers in various areas, including those working in clinical research. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, ALINE R. R.; PAGAN, LUANA U.; DAMATTO, RICARDO L.; CEZAR, MARCELO D. M.; BONOMO, CAMILA; GOMES, MARIANA J.; MARTINEZ, PAULA F.; GUIZONI, DANIELE M.; CAMPOS, DIJON H. S.; DAMATTO, FELIPE C.; et al. Effects of growth hormone on cardiac remodeling and soleus muscle in rats with aortic stenosis-induced heart failure. ONCOTARGET, v. 8, n. 47, p. 83009-83021, OCT 10 2017. (09/54102-3, 12/50512-5)

CEZAR, MARCELO D. M.; DAMATTO, RICARDO L.; PAGAN, LUANA U.; LIMA, ALINE R. R.; MARTINEZ, PAULA F.; BONOMO, CAMILA; ROSA, CAMILA M.; CAMPOS, DIJON H. S.; CICOGNA, ANTONIO C.; GOMES, MARIANA J.; et al. Early Spironolactone Treatment Attenuates Heart Failure Development by Improving Myocardial Function and Reducing Fibrosis in Spontaneously Hypertensive Rats. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v. 36, n. 4, p. 1453-1466, 2015. (09/54102-3)