Acquisition of a confocal microscope for the objective evaluation of markers in anatomical pathology

• Grant number: 09/54204-0
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2010
• End date: July 31, 2012
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Fernando Augusto Soares
• Grantee: Fernando Augusto Soares
• Host Institution: Hospital A C Camargo. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

One of the most powerful molecular pathology tools used in oncology is the identification of markers of the development, progression, and biological behavior of tumors, markers that can also predict the treatment response. Although the investigation of gene expression has identified a large number of genes related to these biological processes, those findings need to be validated. One of the most commonly used methods is the identification of transcripts and proteins by in situ hybridization, immunohistochemistry or immunofluorescence. These methods are extremely valuable but sometimes lack objective analysis. The identification of markers can reveal anomalous intracellular localizations that can be related to their intrinsic activity, just as the relationship between two markers in a signaling pathway can be important for understanding cell function. Major technological advances have been made in the objective analysis of the localization of relationships between two or more markers, as well as in the recognition of cellular colocalization, by confocal microscopy. This request aims to add quality to anatomopathological research projects at various facilities by acquiring a nextgeneration confocal microscope. (AU)