Acquisition of a system of transverse microradiography for studies on tooth demineralization and remineralization, associated or not to adhesive restorations

• Grant number: 09/53849-8
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2010
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Ana Carolina Magalhães
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

To compare the properties of artificial carious lesions, a combination of the determination of crosssectional hardness (mechanical test) and transverse microradiography (determination of mineral content and lesion depth) must be applied to each sample evaluated. Although there have been few studies comparing microhardness testing and microradiography, some degree of correlation has been demonstrated. However, the equations for converting microhardness data into a mineral content profile differ considerably. This indicates that the calculation of mineral content based on hardness values might not be a viable technique. Although microhardness testing is widely employed in Brazil, it has certain limitations: influence of the quantity of organic material and water present in the tissue during the indentation process (critical for the dentin); lack of sensitivity for identifying alterations at the first 50 µm of the subsurface of the dental tissue, due to the size of the indentation. Additionally, with this methodology, it is not possible to distinguish between surface and subsurface lesions, calculate the loss or gain of mineral content, and establish a minimum hardness value for the healthy tissue. In contrast, transverse microradiography is considered the gold standard for the analysis of carious lesions produced in vitro or in situ, since the mineral content values for the dental enamel are established and on the basis of which it is possible to calculate gains and losses in terms minerals. However, there is no transverse microradiography equipment available in Brazil. Therefore, the acquisition of this technology will benefit researchers throughout the country, reducing costs by eliminating the need to send graduate students abroad and significantly increasing the number of research about dental caries. Consequently, this technological knowledge will soon bring major benefits to the Brazilian population. In addition, this equipment will have a positive impact on competitiveness at the international level for the publication of scientific articles. The equipment will be applied in studies involving mineralized tissues (teeth and bones), making it possible for researchers at Bauru School of Dentistry to establish partnerships with those in various other areas of knowledge and universities. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE AMOEDO CAMPOS VELO, MARILIA MATTAR; MAGALHAES, ANA CAROLINA; SHIOTA, AYMEE; HERRERA FARHA, ANA LAURA; GRIZZO, LARISSA TERCILIA; HONORIO, HEITOR MARQUES; WANG, LINDA. Profile of high-fluoride toothpastes combined or not with functionalized tri-calcium phosphate on root dentin caries control: An in vitro study. AMERICAN JOURNAL OF DENTISTRY, v. 31, n. 6, p. 290-296, DEC 2018. (15/00817-2, 09/53849-8)

ARTHUR, RODRIGO ALEX; MARTINS, VANESSA BALBE; DE OLIVEIRA, CAROLINE LONGARAY; BRANCO LEITUNE, VICENTE CASTELO; COLLARES, FABRICIO MEZZOMO; MAGALHAES, ANA CAROLINA; MALTZ, MARISA. Effect of over-the-counter fluoridated products regimens on root caries inhibition. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 10, p. 1588-1594, OCT 2015. (09/53849-8)

ALANIA, YVETTE; NATALE, LIVIA C.; NESADAL, DOUGLAS; VILELA, HANDIALLY; MAGALHAES, ANA C.; BRAGA, ROBERTO R.. In vitro remineralization of artificial enamel caries with resin composites containing calcium phosphate particles. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 107, n. 5, p. 1542-1550, JUL 2019. (12/25253-6, 09/53849-8)