Evaluation on the interaction between two osteogenic proteins and different bone grafts in the bone healing process of calvarial tissue in Wistar rats

• Grant number: 11/01512-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2011
• End date: May 31, 2013
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: João Paulo Mardegan Issa
• Grantee: João Paulo Mardegan Issa
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Bruna Gabriela dos Santos Kotake ; Fernando José Dias ; Joaquim Coutinho Netto ; Mamie Mizusaki Iyomasa ; Mirella Lemos de Queiróz Tavares ; Yamba Carla Lara Pereira

Abstract

With the discovery of proteins with osteogenic potential, it was radically changed the thinking about the bone induction, these proteins are multifunctional and with wide effects on cell growth and differentiation in many organisms. The role of BMPs in critical bone defects has been studied in the literature, but it has not been examined in large extension. This study aims to evaluate the bone healing process in critical bone defects performed in rat skulls, using different types of bone grafts, the osteoinductive protein rhBMP-2, and the association between these grafts and this protein, by histomorphometric, immunohistochemical methods, and statistical analysis. It will be used 112 Wistar rats, male (250 g) to be divided into 8 groups, and a period of time until the sacrifice of 4 and 6 weeks. The groups are the following: 1) particulate autologous bone graft; 2) particulate allografts, 3) heterologous bone graft (lyophilized bovine bone), 4) particulate autologous bone graft plus 5 µg rhBMP-2, 5) particulate allografts plus 5 µg rhBMP-2, 6) heterologous bone graft (lyophilized bovine bone) plus 5 µg rhBMP-2, 7) 5 µg rhBMP-2, 8) only critical bone defect. The results for new bone formation will be evaluated by histomorphometric and immunohistochemical methods, and the resulting data from these assessments will be submitted to statistical analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ISSA, JOAO PAULO; GONZAGA, MILIANE; KOTAKE, BRUNA GABRIELA; DE LUCIA, CONRADO; ERVOLINO, EDILSON; IYOMASA, MAMIE. Bone repair of critical size defects treated with autogenic, allogenic, or xenogenic bone grafts alone or in combination with rhBMP-2. Clinical Oral Implants Research, v. 27, n. 5, p. 558-566, MAY 2016. (11/01512-0)

GONZAGA, MILIANE GONCALVES; DOS SANTOS KOTAKE, BRUNA GABRIELA; TOCCHINI DE FIGUEIREDO, FELLIPE AUGUSTO; FELDMAN, SARA; ERVOLINO, EDILSON; GORITA DOS SANTOS, MARIA CECILIA; MARDEGAN ISSA, JOAO PAULO. Effectiveness of rhBMP-2 association to autogenous, allogeneic, and heterologous bone grafts. MICROSCOPY RESEARCH AND TECHNIQUE, v. 82, n. 6, p. 689-695, JUN 2019. (11/01512-0)