Functional analysis of Pou5f1 (Oct4) gene marker of stem/progenitor cells in cultured tumor pituitary cell lines

• Grant number: 11/03622-7
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2011
• End date: May 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Luciani Renata Silveira de Carvalho
• Grantee: Luciani Renata Silveira de Carvalho
• Host Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Ricardo Vieira Araujo

Abstract

The maintenance of tissue in the adult organism requires a constant supply of new cells to replace cells lost to stress, injury or natural death. The cell replacement is possible through the activity attributed to tissue-specific stem cells. The anterior pituitary is an important regulatory role in the metabolism of the adult organism by regulating the hormone production. The cellular composition of the gland undergoes changes in response to hormonal signals, for example, the increase of the gland during puberty and pregnancy. Besides the known role of stem cells during embryogenesis pituitary and its maintenance in adulthood. Another emerging concept of stem cell biology is its connection with carcinogenesis, underscored by the consistent presence of a compartment of undifferentiated cells connected with the initiation, growth and maintenance of tumors such as breast, glioblastomas, among others. It was recently shown in humans that ACTH-producing tumors have an increased expression of the gene Pou5f1 (Oct4), compared to normal pituitary gland. This gene is expressed in embryonic stem cells and stem / progenitor cells of many tumors. Therefore, the objective of this study is to evaluate the role of gene Pou5f1 (Oct4) in proliferation and hormonal phenotype of tumor cells from pituitary cell cultures of mouse and rat. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHANG, CLAUDIA VEIGA; ARAUJO, RICARDO VIEIRA; CIRQUEIRA, CINTHYA SANTOS; GOMES CANI, CAROLINA MARIA; MATUSHITA, HAMILTON; SPERLING CESCATO, VALTER ANGELO; BARISSON VILLARES FRAGOSO, MARIA CANDIDA; BRONSTEIN, MARCELLO DELANO; NOGUEIRA ZERBINI, MARIA CLAUDIA; MENDONCA, BERENICE BILHARINHO; et al. Differential Expression of Stem Cell Markers in Human Adamantinomatous Craniopharyngioma and Pituitary Adenoma. Neuroendocrinology, v. 104, n. 2, p. 183-193, 2017. (11/03622-7)