Chromosome estudy in tribe Dalbergieae sensu Klitgaard & Lavin (2005), with emphasis in "informal" clade Dalbergia (Leguminosae, Papilionoideae) and the application of molecular techniques to obtain new probes for DNA in situ hybridization

• Grant number: 10/19132-6
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2011 - November 30, 2013
• Field of knowledge: Biological Sciences - Botany - Plant Taxonomy
• Principal Investigator: Eliana Regina Forni Martins
• Grantee: Eliana Regina Forni Martins
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Paula de Moraes ; Caroline Do Amaral Polido

Abstract

The Dalbergieae sensu Klitgaard & Lavin (2005) tribe presents 49 genera and nearly 1,325 species distributed mainly on the tropical regions. Based on recently molecular studies, its circumscription was hardly altered, resulting on divergences between morphological and phylogenetically studies. In this way, the present study aims to help the taxonomic and evolutionary discussion on this tribe, with emphasis on the genus Aeschynomene L. by studying the karyotype of species from diverse vegetal formation, mainly from Rocky outcrop ("Cerrado") and Chaco Sul-matogrossense. In this study will be determined the chromosome number and elaborated the karyotype based on conventional staining, chromosome banding (CMA and DAPI) and in situ hybridization. Different cytomolecular markers will be tested, as ribosomal DNA 5S and 45S, satellite and microsatellites DNA sequences, beyond BACs from Phaseolus vulgaris L. The chromosome analysis data will be used on cytotaxonomic discussion on Aeschynomene genus, supporting the genus delimitation analysis and its evolutionary aspects. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POLIDO, C. A.; MANTELLO, C. C.; MORAES, A. P.; SOUZA, A. P.; FORNI-MARTINS, E. R.. Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization. Genetics and Molecular Research, v. 13, n. 4, p. 10390-10397, 2014. (11/22215-3, 10/19132-6)