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Sequencing of bovine expressed sequence tags (BEST)

Grant number: 02/03504-5
Support type:Research Grants - Research Partnership for Technological Innovation - PITE
Duration: February 01, 2003 - July 31, 2004
Field of knowledge:Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
Principal researcher:Luiz Lehmann Coutinho
Grantee:Luiz Lehmann Coutinho
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Company: Angus Bela Vista Pecuária Ltda
City: Piracicaba

Abstract

The main objective of this project was the identification of bovine genes with a potential for use in the development of new products and technologies to be exploited commercially. In parallel, the information created while the work was in progress was of inestimable value to the scientific community, notably in the areas of zootechnics, biology, genetic and veterinary medicine, among others. For this purpose, approximately 100 thousand expressed sequences (ESTs) were analyzed, obtained from the sequencing of libraries of cDNA, prepared with the messenger RNA extracted from different organs and bodily tissue (such as testicles, skin, muscle tissue and adipose tissue). The production of libraries of cDNA followed the strategy used by the Sucest project (Sugarcane EST Project), in which the fragments of cDNA produced with the SuperScript Plasmid System kit (Gibco-BRL) are selected by size in seraphose column and subsequently cloned in the vectors pSport-1 and 6, which were transferred by cells of Escherichia coli (DH10B strain) by the technique of electroporation. (AU)

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