Soybean and Fish oil mixture increases IL-10, protects against DNA damage and decreases colonic inflammation in rats with dextran sulfate sodium (DSS) colitis

• Grant number: 10/16673-6
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: November 01, 2010
• End date: April 30, 2011
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Vera Lucia Flor Silveira
• Grantee: Vera Lucia Flor Silveira
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

It was investigated whether dietary polyunsaturated fatty acids (PUFA) could influence colonic injury, tissue DNAdamage, cytokines and myeloperoxidase activity (MPO) and plasma corticosterone in DSS-induced colitis rats. Maleweaning Wistar rats were fed for 47 days with an AIN-93 diet with control (C), fish (F) or a mixture of fish and soybean oil(SF). The colitis was induced from day 36 until day 42 by 3% DSS in drinking water. On day 48, blood samples werecollected for corticosterone determination. The distal colon was excised for histological analysis and to quantify thecytokine (IL-4, IL-10 and INF-³), MPO and DNA damage. The disease activity index (DAI) was recorded daily during colitisinduction. The DAI, MPO, histological analyses showed decreases only in the SF group compared with the C group. IL-10 was increased and DNA damage was reduced in the groups F and SF, and an inverse correlation between thesevariables was found. There were no differences in corticosterone, IFN-³ and IL-4 levels. Soybean and fish oil mixture maybe effective in improving colonic injury and DNA damage, and it could be an important complementary therapy in UCto reduce the use of anti-inflammatory drugs and prevent colorectal cancer. (AU)