Osmotic stress in the control of direct somatic embryogenesis em genotypes of Coffea arabica

• Grant number: 11/10090-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2011
• End date: August 31, 2013
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Julieta Andrea Silva de Almeida
• Grantee: Julieta Andrea Silva de Almeida
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

The coffee market has high demand for hybrids of Coffea arabica L. that have good quality drink. However, the multiplication of these hybrids must be vegetatively because plants from germination of seeds lose the genetic characteristics of the hybrid, due to genetic segregation. Somatic embryogenesis (ES) allows the vegetative propagation of these hybrids. This process occurs in two ways, indirect somatic embryogenesis (ESI), consisting of the stages of embryogenesis and organogenesis, somatic embryogenesis or direct (ESD) at one stage. The comparison between the two approaches indicates that the ESD is more advantageous than ESI, since the embryos are formed with reduced inputs, labor and time. However, genotypes of C. arabica not respond efficiently to ESD, this means they have no or low production of somatic embryos are formed and the long period of time. Thus, there is a need for more efficient implementation of ESD in genotypes of arabica coffee. Stressors such as osmotic has been found to increase the capacity of ES in different plant species. Thus, it is intended to define the effect of osmotic stress in controlling the occurrence of the ESD in three hybrids of C. arabica, with good sensory quality, and cv Mundo Novo, in order to increase the production of somatic embryos, reduce your training time, develop a more efficient protocol for the implementation of ESD in this species and the production of seedlings of selected hybrids that will later be subjected to the condition field for evaluation of agronomic performance. For this, we performed six experiments, five with the induction of osmotic stress in leaf explants and on the evaluation of regenerated seedlings under this condition. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, IVANILDA DOS SANTOS; BARBOSA CARMAZINI, VALERIA CRISTINA; DOS SANTOS, COSME DAMIAO; SILVA DE ALMEIDA, JULIETA ANDREA. 2-Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.. Ciência Rural, v. 48, n. 11, 2018. (11/10090-1)

JULIETA ANDREA SILVA DE ALMEIDA; WILIELLE CRISTINA FREITAS NEOFTI; GERSON SILVA GIOMO. Intensidade do potencial osmótico no controle da embriogênese somática direta em cafeeiro. Pesquisa Agropecuária Brasileira, v. 57, 2022-11-07. (11/10090-1)

IVANILDA DOS SANTOS ALVES; VALÉRIA CRISTINA BARBOSA CARMAZINI; COSME DAMIÃO DOS SANTOS; JULIETA ANDREA SILVA DE ALMEIDA. 2- Isopenteniladenina na capacidade de indução da embriogênese somática direta de Coffea arabica L.. Ciência Rural, v. 48, n. 11, 2018-11-14. (11/10090-1)