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Postoperative pain and inflammatory response, evaluated by electrophoretic separation of serum proteins, in dogs undergoing ovariohysterectomy performed by laser or conventional minimally invasive surgical technique

Grant number: 11/02968-7
Support type:Regular Research Grants
Duration: September 01, 2011 - February 28, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Stelio Pacca Loureiro Luna
Grantee:Stelio Pacca Loureiro Luna
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Assoc. researchers:Alfredo Feio da Maia Lima ; Elizabeth Moreira dos Santos Schmidt ; José Jurandir Fagliari ; Marilda Onghero Taffarel


Ovariosalpingohisterectomy (OSH) is the most common procedure for surgical contraception in female dogs and cats. Despite being a relatively safe, effective and inexpensive technique, trans and postoperative complications and pain related to inflammation may be observed. These complications may be minimized by the use of minimally invasive techniques and other alternative methods. In this context laser surgery may be an excellent option for reducing the risk of infection, inflammation and pain, compared to the conventional surgical procedure. The acute phase proteins are important biomarkers of inflammation. Based on the above considerations, the aim of this study is to compare postoperative pain and inflammatory response by measuring serum protein concentrations, using the SDS-PAGE technique, in bitches undergoing OSH by laser or conventional surgical technique. The study hypothesis is that laser surgery will trigger less inflammatory response and postoperative pain than conventional surgery. Forty dogs will be submitted to OSH; 20 will be submitted to conventional minimally invasive technique (GC) and 20 laser surgery (GL). Postoperative pain will be evaluated by Glasgow Composite Measure Pain Scale and interactive and dynamic visual analogue scale, in a blind study. Blood counts and acute phase serum proteins will be measured immediately before and at 12, 24, 48 and 72 hours and 7 days after surgery. Profile analysis will be performed for statistical analysis, with a significance level of 5%. (AU)