Relative importance of glial reactivity and expression of MHC Class I for the maintenance of neuronal circuits in vitro

• Grant number: 11/08123-9
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2011
• End date: November 30, 2013
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Alexandre Leite Rodrigues de Oliveira
• Grantee: Alexandre Leite Rodrigues de Oliveira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Rafaela Chitarra Rodrigues Hell ; Renata Graciele Zanon

Abstract

Astrocytes become reactive after lesion of the nervous system after trauma or during pathological states. Since such reactivity may be harmful to the neural tissue, the present work aims to regulate GFAP and MHC I expression in order to modulate the astroglial response. For this purpose, RNA interference will be used in astrocyte and neuronal cell co-cultures. The results will be analyzed by immunohistochemistry and RT-PCR. Transmission electron microscopy will also be applied. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOMBEIRO, ANDRE LUIS; RODRIGUES HELL, RAFAELA CHITARRA; SIMOES, GUSTAVO FERREIRA; DE CASTRO, MATEUS VIDIGAL; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Importance of major histocompatibility complex of class I (MHC-I) expression for astroglial reactivity and stability of neural circuits in vitro. Neuroscience Letters, v. 647, p. 97-103, APR 24 2017. (11/08123-9, 14/06892-3, 10/05370-2)