Study on the mobilization, activation, and apoptosis of bone marrow cells in a rat model of brain death

• Grant number: 11/22803-2
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2012
• End date: March 31, 2014
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Paulina Sannomiya
• Grantee: Paulina Sannomiya
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

It has been previously shown that brain death itself induces hypoperfusion at mesenteric microcirculation associated to a pronounced reduction in endogenous corticosterone levels leading to increased local inflammation and organ dysfunction in a rat model of brain death. These events occur paradoxically associated with induced leucopenia after brain damage. This study aims to investigate bone marrow cells mobilization, activation, and the apoptotic process in a rat model of brain death. The design includes the investigation of: (1) bone marrow and white blood cell counts; (2) bone marrow histology; (3) stem cell markers by flow cytometry; (4) apoptosis and necrosis by flow cytometry; (5) expression of adhesion molecules (L-selectin, CD11a, and CD11b/c) on bone marrow and white blood cells by flow cytometry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENEGAT, LAURA; SIMAS, RAFAEL; CALIMAN, JULIA M.; ZANONI, FERNANDO L.; JACYSYN, JACQUELINE F.; DA SILVA, LUIZ FERNANDO F.; BORELLI, PRIMAVERA; MOREIRA, LUIZ FELIPE P.; SANNOMIYA, PAULINA. Evidence of bone marrow downregulation in brain-dead rats. International Journal of Experimental Pathology, v. 98, n. 3, p. 158-165, JUN 2017. (11/22803-2)