Prevalence of Aspergillus species in samples from clinical/environmental origin and analysis of their susceptibility profile to six antifungals

• Grant number: 12/01134-8
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: May 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Arnaldo Lopes Colombo
• Grantee: Arnaldo Lopes Colombo
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Sarah Santos Gonçalves

Abstract

Currently, species of Aspergillus genera are responsible by the second cause of invasive fungal infection in tertiary hospital. In the clinical practice, those infections are difficult to diagnostic and the delayed treatment is very frequent. Few medical centers have the capacity to perform the accurate identification of species in this genus of fungi. The resistance cases to antifungal are not common in the contemporaneous medicine, but the number of resistance cases and treatment failure have increased in the last decades. There is no data in Brazil about the prevalence of different species of Aspergillus and its susceptibility profile. This study has as major objective to analyze distribution from clinical/environmental samples of Aspergillus and evaluate occurrence of resistant strains to triazolics. The identification of Aspergillus species will be held by polyphasic approach, including micromorphology analysis, biochemical tests and sequencing of three genes. Moreover, will be held the characterization of antifungal susceptibility by three different methods: microdilution in broth, diffusion disk and E-test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NEGRI, C. E.; GONCALVES, S. S.; XAFRANSKI, H.; BERGAMASCO, M. D.; AQUINO, V. R.; CASTRO, P. T. O.; COLOMBO, A. L.. Cryptic and Rare Aspergillus Species in Brazil: Prevalence in Clinical Samples and In Vitro Susceptibility to Triazoles. Journal of Clinical Microbiology, v. 52, n. 10, p. 3633-3640, OCT 2014. (12/19103-1, 12/01134-8, 12/01548-7)

GONCALVES, SARAH S.; STCHIGEL, ALBERTO M.; CANO, JOSEP; GUARRO, JOSEP; COLOMBO, ARNALDO L.. In Vitro Antifungal Susceptibility of Clinically Relevant Species Belonging to Aspergillus Section Flavi. Antimicrobial Agents and Chemotherapy, v. 57, n. 4, p. 1944-1947, APR 2013. (12/01134-8)